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The Tachykinin Receptors pp 39–66Cite as

Biochemical Methods and Assays

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Part of the book series: The Receptors ((REC))

Abstract

The tachykinins are a family of peptides that share the common C-terminal sequence These peptides exert their biological effects via three pharmacologically and molecular biologically well-defined receptors (see other chapters in this volume), the receptors. Substance P (SP) binds preferentially to the receptor, neurokinin A (NKA) to the receptor, and neurokinin B (NKB) to the receptor. Among other things, the number of receptor sites in tissues/cells and the affinities of compounds/peptides can be measured readily in receptor binding assays that exist for each individual tachykinin receptor. In this chapter, we will describe the development of these assays and the various factors that can influence their outcome. For reasons of clarity, the current tachykinin receptor nomenclature has been used throughout the chapter.

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Authors
  1. Paul L M. van Giersbergen
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  2. Stephen H. Buck
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  1. Biopharm Consultists, Tucson, AZ, USA

    Stephen H. Buck

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van Giersbergen, P.L.M., Buck, S.H. (1994). Biochemical Methods and Assays. In: Buck, S.H. (eds) The Tachykinin Receptors. The Receptors. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0301-8_3

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  • DOI: https://doi.org/10.1007/978-1-4612-0301-8_3

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