PCR Amplification of Viral DNA and Viral Host Cell mRNAs in Situ
The hallmark and power of in situ hybridization methodology are its ability to determine the levels of gene expression in an individual cell in a population or determine which cells in a population have acquired new genes by infection or other processes. In this way one can gain insight into how levels of gene expression can vary within a population with the same complement of genes, how different genes within a cell are coordinately regulated, and how newly introduced genes are regulated and interact with genes in the host cell. In contrast to other techniques that measure the average number of molecules per cell in the population, in situ analyses measure the number of molecules in a particular cell in a particular spatial and temporal context. With double-labeling techniques, one can also appreciate, for example, the relative abundancy of specific nucleic acid and protein in the same cell.
KeywordsInfected Cell Single Prime Pair Visna Virus Individual Prime Pair PDGF mRNA
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- Bitterman P, Haase A, Embretson J, Peterson M, Harman K, Jessurum J (1993): Combination of the polymerase chain reaction with in situ hybridization to define the spatial pattern of mRNA in tissue. Manuscript submitted.Google Scholar
- Embretson J, Zupanocic M, Beneke J, Till M, Wolinsky S, Ribas JL, Burke A, Haase AT (1993): Analysis of human immunodeficiency virus infected tissues by amplification and in situ hybridization reveals latent and permissive infections at single cell resolution. Proc Natl Acad Sci USA 90:357–361.PubMedCrossRefGoogle Scholar