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PCR and Cancer Diagnostics: Detection and Characterization of Single Point Mutations in Oncogenes and Antioncogenes

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Book cover The Polymerase Chain Reaction

Abstract

The theory that the accumulation in a single cell of genetic alterations in some critical genes, the oncogenes and the tumor suppressor genes, is the underlying cause of neoplastic transformation is becoming generally accepted (Fearon and Vogelstein, 1990; Bishop, 1991). Cellular genes whose products are involved in any of the steps of signal transduction leading to cell replication are potential candidates for oncogenes and for tumor suppressor genes. For an extensive review on oncogenes, see Pimentel (1989). For recent reviews on tumor suppressor genes or antioncogenes, see Weinberg (1991) and Marshall (1991a). The balanced interplay between protooncogenes and tumor suppressor genes in cell growth and differentiation is disrupted by cumulative genetic damage and, despite the fail-safe mechanisms provided by natural selection, neoplastic transformation occurs.

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  1. Manuel Perucho
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  1. La Jolla, CA, 92037, USA

    Kary B. Mullis

  2. The Immune Response Corporation, 5935 Darwin Court, Carlsbad, CA, 92008, USA

    François Ferré

  3. Institute for Molecular Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA

    Richard A. Gibbs

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Perucho, M. (1994). PCR and Cancer Diagnostics: Detection and Characterization of Single Point Mutations in Oncogenes and Antioncogenes. In: Mullis, K.B., Ferré, F., Gibbs, R.A. (eds) The Polymerase Chain Reaction. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-0257-8_31

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