Fingerprinting Using Arbitrarily Primed PCR: Application to Genetic Mapping, Population Biology, Epidemiology, and Detection of Differentially Expressed RNAs
Several approaches to genetic mapping and phylogenetic analysis using PCR have been developed over the past few years. The ability to examine specific sequences of DNA from many organisms simultaneously and without the need of standard cloning has streamlined several types of analysis that were already commonly used but rather labor intensive and has also made possible several types of analysis that were previously not possible. For example, RFLP analysis using Southern blotting has been the principal tool for chromosomal mapping in mammals and plants and, in some situations, has been replaced by PCR-based methods (e.g., Litt and Luty, 1989; Nelson et al., 1989; Orita et al., 1990; Sinnet et al., 1990; Ledbetter et al., 1990). Methods based on PCR have also been developed for studies in phylogenetics and population biology.
KeywordsGenetic Mapping tRNA Gene Recombinant Inbred Borrelia Burgdorferi High Stringency
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