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The Polymerase Chain Reaction pp 201–213Cite as

Birkhäuser

PCR and DNA Sequencing

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Abstract

The development of protocols for the direct DNA sequence analysis of PCR products and the use of PCR to facilitate conventional sequencing strategies have been the focus of investigators since 1986. Direct PCR sequencing enables rapid and precise determination of sequence identity and variation, which is useful in most aspects of molecular biology and for diagnostic genetic applications. In a more general way, PCR improves the ease and capacity of all DNA sequencing activities, by simplifying the screening, preparation, and manipulation of DNA templates.

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Authors
  1. Bjorn Andersson
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  2. Richard A. Gibbs
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Editor information

Editors and Affiliations

  1. La Jolla, CA, 92037, USA

    Kary B. Mullis

  2. The Immune Response Corporation, 5935 Darwin Court, Carlsbad, CA, 92008, USA

    François Ferré

  3. Institute for Molecular Genetics, Baylor College of Medicine, One Baylor Plaza, Houston, TX, 77030, USA

    Richard A. Gibbs

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© 1994 Springer Science+Business Media New York

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Andersson, B., Gibbs, R.A. (1994). PCR and DNA Sequencing. In: Mullis, K.B., Ferré, F., Gibbs, R.A. (eds) The Polymerase Chain Reaction. Birkhäuser, Boston, MA. https://doi.org/10.1007/978-1-4612-0257-8_17

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  • DOI: https://doi.org/10.1007/978-1-4612-0257-8_17

  • Publisher Name: Birkhäuser, Boston, MA

  • Print ISBN: 978-0-8176-3750-7

  • Online ISBN: 978-1-4612-0257-8

  • eBook Packages: Springer Book Archive

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