The object of tissue culture is to maintain viable single cells (cell culture) or a functional unit of cells (organ culture) outside of their normal multicellular organism. The pioneers in this technique, who removed cells and organs, kept them viable, and were able to make them multiply and grow, had a revolutionary impact on science. Their work gave birth to the new discipline of cell biology which has advanced almost explosively during the past half century Cell biology advances have gone hand in hand with the ever-increasing ability to isolate, maintain, and examine increasing numbers of cell types. Today, tissue culture is not just for cell biologists, but is an adjunct to virtually every discipline of the biological sciences.
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