Designing Time-of-Flight Mass Spectrometers for the Future: Multichannel Recording of Peptide Amino Acid Sequences

  • Robert J. Cotter
  • Timothy J. Cornish
  • Marcela Cordero

Abstract

Mass spectrometry has played an important role in research in the health and life sciences for many years. Identification and quantitation of the metabolites of new drugs, for example, has generally involved the use of combined gas chromatography/mass spectrometry (GCMS). However, in recent years, the introduction of new ionization techniques such as plasma desorption mass spectrometry (PDMS), fast atom bombardment (FAB), electrospray ionization (ESI) and matrix-assisted laser desorption / ionization (MALDI) has extended the analytical capabilities of mass spectrometers. The samples now amenable to these new techniques include peptides and proteins, carbohydrates and glycopeptides, and oligonucleotides. Double-focusing sector instruments and quadrupole mass filters continue to be the most commonly used mass analyzers. Because they record mass spectra by scanning the mass range, they are most appropriately used for methods (such as FAB and ESI) which produce ions continuously. In addition, sector and quadrupole analyzers are easily combined to form tandem (or hybrid) configurations that can provide detailed structural analysis from samples that may include a mixture of analytes. Thus, combinations of FAB with a four-sector mass analyzer or ESI with a triple quadrupole analyzer have become the major high performance instruments for the amino acid sequence analysis of peptides and proteins.

Keywords

Carbohydrate Argon Helium Electrophoresis Disulfide 

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Robert J. Cotter
    • 1
  • Timothy J. Cornish
    • 1
  • Marcela Cordero
    • 1
  1. 1.Middle Atlantic Mass Spectrometry Laboratory, Department of Pharmacology and Molecular SciencesThe Johns Hopkins University School of MedicineBaltimore

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