Use of KLa as a Criterion for Scaling Up the Inulinase Fermentation Process

  • Adalberto PessoaJr.
  • Michele Vitolo
  • Helmut Hustedt
Part of the ABAB Symposium book series (ABAB, volume 57/58)

Abstract

The scale-up of inulinase production in aerated cultures of Candida kefyr DSM 70106 was studied, taking into account the criterion of maintaining K L a constant. The culture was carried out batchwise, in a 15-L fermentor, with K L a varying from 25 to 199 h-1. The highest inulinase production was attained with an initial K L a value of 46 h-1. A large scale fermentation (300-L fermentor) was performed using identical culture medium conditions. The responses obtained for the bench and scaled-up experiments showed similar behaviors, and the results were, respectively, 0.60 and 0.58 U•mL-1•h-1 for productivity and 43.0 and 41.5 U•mL-1 for activities.

Index Entries

Scale-up inulinase KLa fermentation Candida kefyr 

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Adalberto PessoaJr.
    • 1
  • Michele Vitolo
    • 2
  • Helmut Hustedt
    • 3
  1. 1.Departamento de Biotecnologia/FAENQU1L-12.600–000-Lorena/SPBrazil
  2. 2.Uniuersidade de Sao Paulo/FCF-CP. 66083-Sao Paulo/SPBrazil
  3. 3.Gesellschaft für Biotechnologische Forschung/GBF Mascheroder WegGermany

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