Abstract
A continuous biparticle fluidized-bed reactor (BFBR) is developed for the simultaneous fermentation and recovery of lactic acid. In this processing scheme, bacteria are immobilized in gelatin beads and are fluidized in a columnar reactor. Solid particles (weak-base resin IRA-35) with sorbent capacity for the product are introduced at the top of the reactor and fall countercurrently to the biocatalyst, effecting in situ removal of the inhibitory lactic acid while also controlling reactor pH at optimal levels. One-week-long fermentation trials using immobilized Lactobacillus delbreuckii with sorbent addition demonstrated a volumetric productivity (6. 9 g/L. h) at least 16-fold higher than that of a free-cell batch fermentation with base pH control and identical biomass concentration and medium composition. Regeneration of the loaded sorbent from the BFBR has effected a 35-fold concentration of lactic acid compared with original levels in the fermentation broth (70 vs 2 g/L). Lactic acid concentrations as high as 610 g/L have been observed when the loading solution contained 50 g/L lactic acid. Rich medium formulations did not seem to increase BFBR performance. The benefits of this reactor system, as opposed to conventional batch fermentation, are discussed in terms of productivity and process economics.
Author to whom all correspondence and reprint requests should be addressed.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Atkinson, B. and Mavituna, F. (1991) Biochemical Engineering and Biotechnology Handbook, 2nd ed. Stockton, New York.
Lipinsky, E. S. and Sinclair, R. G. (1986)Chem. Eng. Prog. 82, 26–32.
Chemical Marketing Reporter (1994), March 14, pp. 28–36.
Millis, J. (1993) Am. Chem. Soc. 38, 297–299.
Ohleyer, E., Blanch, H. W., and Wilke, C. R. (1985) Appl. Biochem. Biotechnol. 11, 317–331.
Evangelista, R. L., Mangold, A. J., and Nikolov, Z. L. (1994) Appl. Biochem. Biotechnol. 45, 131–144.
Mercier, P., Yerushalmi, L., Rouleau, D., and Dochain, D. (1992), J. Chem. Tech. Biotechnol. 55, 111–121.
Buchta, K. (1983), in Biotechnology: A Comprehensive Treatise Biomass, Microorganisms for Special Applications, Microbial Products I, Energy from Renwable Resources, Dellweg, H., ed., Verlag Chemie, Weinheim, Germany, 410–417.
Mark, H. F, Othmer, D. F., Overberger, C. G., and Seaborg, G. T., eds. (1981) Kirk-Othmer Encyclopedia of Chemical Technology, 3rd ed., vol. 13, Wiley, New York.
King, C. J. (1992) Chem. Tech. 22, 285–291.
Yeh, P. L. H., Bajpai, R. K., and Iannotti, E. L. (1991) J. Fermentation Bioeng. 71, 75–77.
Kuhn, R., Peretti, S., and Ollis, D. (1993) Appl. Biochem. Biotechnol. 39/40, 401–413.
Kaufman, E. N., Cooper, S. P., Clement, S. L., and Little, M. H. (1995) Appl. Biochem. Biotechnol. 51, 605–620.
Melzoch, K. and Konopaskova, L. (1993) Biotechnol. Lett. 15, 517–520.
Andrews, G. F. and Fonta, J. P. (1989) Appl. Biochem. Biotechnol. 20, 375–390.
Stenroos, S. L., Linko, Y. Y., and Linko, P. (1982) Biotechnol. Lett. 4, 159–164.
Guoqiang, D., Kaul, R., and Mattiasson, B. (1991) Appl. Microbiol. Biotechnol. 36, 309–314.
Hang, Y. D., Hamamci, H., and Woodams, E. E. (1989) Biotechnol. Lett. 11, 119–120.
Yabannavar, V. M. and Wang, D. I. C. (1991) Biotechnol. Bioeng. 37, 1095–1100.
Vick Roy, T. B., Blanch, H. W., and Wilke, C. R. (1982) Biotechnol. Lett. 4, 483–488.
Shi, Z., Shimizu, K., Iijima, S., Morisue, T., and Kobayashi, T. (1990), J. Fermentation Bioeng. 70, 415–419.
Xavier, A. M. R. B., Goncalves, L. M. D., Moreira, J. L., and Carrondo, M. J. T. (1995) Biotechnol. Bioeng. 45, 320–327.
Kulozik, U., Hammelehle, B., Pfeifer, J., and Kessler, H. G. (1992) J. Biotechnol. 22,107–116.
Venkatesh, K. V., Okos, M. R., and Wankat, P. C. (1993) Process Biochem. 28, 231–241.
Seevaratnam, S., Holst, J. O., Hjorleifsdottir, S., and Mattiasson, B. (1991) Bioprocess Eng. 6, 35–41.
Martin, M. S., Pazos, C., and Coca, J. (1992) J. Chem. Tech. Biotechnol. 54, 1–6.
Siebold, M., Frieling, P. V., Joppien, R., Rindfleisch, D., Schugerl, K., and Roper, H. (1995) Process Biochem. 30, 81–95.
Garcia, A. A. (1991) Biotechnol. Prog. 7, 33–42.
Galliot, F. P., Gleason, C., Wilson, J. J., and Zwarick, J. (1990) Biotechnol. Prog. 6, 370–375.
Srivastava, A., Roychoudhury, P. K., and Sahai, V. (1992) Biotechnol. Bioeng. 39, 607–613.
Davison, B. H. and Scott, C. D. (1992) Biotechnol. Bioeng. 39, 365–368.
Davison, B. H. and Thompson, J. E. (1992) Appl. Biochem. Biotechnol. 34, 431–439.
Godia, F., Casas, C., and Sola, C. (1987) Process Biochem. 22, 43–48.
Davison, B. H. and Scott, C. D. (1988) Appl. Biochem. Biotechnol. 18, 19–34.
Kaufman, E. N., Cooper, S. P., and Davison, B. H. (1994) Appl. Biochem. Biotechnol. 45, 545–554.
Scott, C. D. (1987) Ann. NY Acad. Sci. 501, 487–493.
Major, N. C. and Bull, A. T. (1989) Biotechnol. Bioeng. 34, 592–599.
Ernst, E. E. and McQuigg, D. W. (1992) in AICHE 1992 National Meeting, Miami Beach, FL.
Nakagawa, M., Nakamura, I., and Kobayashi, T. (1975) J. Fermentation Technol. 53, 127–134.
Yang, C. W., Lu, Z., and Tsao, G. T. (1995) Appl. Biochem. Biotechnol. 51, 57–71.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Kaufman, E.N., Cooper, S.P., Budner, M.N., Richardson, G.R. (1996). Continuous and Simultaneous Fermentation and Recovery of Lactic Acid in a Biparticle Fluidized-Bed Bioreactor. In: Wyman, C.E., Davison, B.H. (eds) Seventeenth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium, vol 57/58. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0223-3_46
Download citation
DOI: https://doi.org/10.1007/978-1-4612-0223-3_46
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-6669-3
Online ISBN: 978-1-4612-0223-3
eBook Packages: Springer Book Archive