Hexokinase Production from S. Cerevisiae

Culture Conditions
  • José Abrahão-Neto
  • Patricía Infanti
  • Michele Vitolo
Part of the ABAB Symposium book series (ABAB, volume 57/58)

Abstract

The effects of pH (4.0, 4.5, or 5.0), temperature (T) (30, 35, or 40°C) and dissolved oxygen (DO) (0.2, 2.0, 4.0, or 6.0 mg O2/L) on hexokinase and invertase formation by yeast were studied. The highest enzyme activities were attained at pH 4.0, DO = 4.0 mg O2/L, and T = 35 or 40°C.

Index Entries

Hexokinase invertase S. cerevisiae baker’s yeast 

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References

  1. 1.
    Entian, K. D. and Zimmermann, F. K. (1980), Mol. Gen. Genet. 177, 345–350.CrossRefGoogle Scholar
  2. 2.
    Baley, R. B. and Woodword, A. (1984), Mol. Gen. Genet. 193, 507–512.CrossRefGoogle Scholar
  3. 3.
    Takeda, M. (1981), Biochem. J. 198, 281–287.Google Scholar
  4. 4.
    Bisson, L. F. and Fraenkel, D. G. (1983), Proc. Natl. Acad. Sci. USA 80, 1730–1734.CrossRefGoogle Scholar
  5. 5.
    Kovák, L., Nelson, B. D., and Ernster, L. (1986), Biochem. Biophys. Res. Commun. 134(1), 285–291.Google Scholar
  6. 6.
    Bergmeyer, H. U. (1984), in Methods of Enzymatic Analysis, vol. 2, 3rd ed., Bergmeyer, H. V., Bergmeyer, J., and Grasl, M., eds., Verlag Chemie, Weinheim, pp. 222,223.Google Scholar
  7. 7.
    Whitaker, J. R. (1991), in Food Enzymology, Elsevier Applied Science, New York.Google Scholar
  8. 8.
    Somogyi, M. (1952), J. Biol. Chem. 195, 19–23.Google Scholar
  9. 9.
    Vitolo, M. and Borzani, W. (1983), Anal. Biochem. 130, 469,470.Google Scholar
  10. 10.
    Borzani, W. (1975), in Biochemical Engineering, Edgard Blucher, Sao Paulo, Brasil.Google Scholar
  11. 11.
    Trumbly, R. J. (1992), Mol. Microbiol. 6, 15–21.CrossRefGoogle Scholar
  12. 12.
    Layman, P. L. (1986), Chem. Eng. News 15, 11–14.CrossRefGoogle Scholar
  13. 13.
    Barlikova, A., Svorc, J., and Miertus, S. (1991), Anal. Chim. Acta 247, 83–87.CrossRefGoogle Scholar
  14. 14.
    Weitzel, G., Pilatus, U., and Rensing, L. (1987), Exp. Cell. Res. 170, 64–79.CrossRefGoogle Scholar
  15. 15.
    Reddy, A. V., Marcoll, R., and Maley, F. (1990), Biochemistry 29(10), 2482–2487.CrossRefGoogle Scholar
  16. 16.
    Patkar, A. and Seo, J. H. (1992), Biotechnol. Bioeng. 40, 103–109.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • José Abrahão-Neto
    • 1
  • Patricía Infanti
    • 1
  • Michele Vitolo
    • 1
  1. 1.Faculdade de Ciências FarmacêuticasUniversity of Sao PauloSão PauloBrasil

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