Abstract
Genetic transformation of two species of diatoms has been accomplished by introducing chimeric plasmid vectors containing a bacterial antibiotic resistance gene driven by regulatory sequences from the acetyl-CoA carboxylase (ACCase) gene from the diatom Cyclotella cryptica. The recombinant DNA integrated into one or more random sites within the algal genome and the foreign protein was produced by the algal transformants. This is the first report of genetic transformation of any chlorophyll c-containing microalgal strain. We are using this system to introduce additional copies of the ACCase gene into diatoms in an attempt to manipulate lipid accumulation in transformed strains.
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Dunahay, T.G., Jarvis, E.E., Dais, S.S., Roessler, P.G. (1996). Manipulation of Microalgal Lipid Production Using Genetic Engineering. In: Wyman, C.E., Davison, B.H. (eds) Seventeenth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium, vol 57/58. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0223-3_20
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DOI: https://doi.org/10.1007/978-1-4612-0223-3_20
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