Downstream Processing of Acetate Fermentation Broths by Nanofiltration

  • In Soo Han
  • Munir Cheryan
Part of the ABAB Symposium book series (ABAB, volume 57/58)


Acetate can be separated from fermentation broths and partially purified by nanofiltration (NF). Membrane performance was a function of pressure, pH, concentration of acetate, temperature, and the presence of other media components. With Nitto-Denko’s NTR729 membrane, average acetate rejection was 60%, glucose rejection was 99%, and flux was 15 L/m2/h at 200 psig, 30°C, pH 5.6, and 20 g/L acetic acid. The best downstream strategy is to clarify the fermentation broth by microfiltration (MF), recycle the cells for improving fermenter productivity, nanofilter the cell-free broth, and then evaporate the permeate. The cost of ceramic MF and NF purification is about $66/t acetate, compared to the cost of potassium acetate ($950/t) or calcium-magnesium acetate ($700/t).

Index Entries

Acetate membrane technology nanofiltration separation 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Parekh, S. R. and Cheryan, M. (1991) Appl. Microbiol. Biotechnol. 36, 384–387.CrossRefGoogle Scholar
  2. 2.
    Parekh, S. R. and Cheryan, M. (1994) Biotechnol. Lett. 16, 139–142.CrossRefGoogle Scholar
  3. 3.
    Witjitra, K., Shah, M. M., and Cheryan, M. (1996) Enzyme Microb. Technol., in press.Google Scholar
  4. 4.
    Cheryan, M. (1986) Ultrafiltration Handbook. Technomic, Lancaster, PA.Google Scholar
  5. 5.
    Parekh, S. R. and Cheryan, M. (1994) Enzyme Microb. Technol. 16, 104–109.CrossRefGoogle Scholar
  6. 6.
    Shah, M. M. and Cheryan, M. (1995) Appl. Biochem. Biotechnol. 51–52, 413–422.CrossRefGoogle Scholar
  7. 7.
    Han, I. S. and Cheryan, M. (1995) J. Membrane Sci. 107, 107–113.CrossRefGoogle Scholar
  8. 8.
    Raman, L. P., Rajagopalan, N., and Cheryan, M. (1994) Chem. Eng. Prog. 90(3), 68–74.Google Scholar
  9. 9.
    Han, I. S. (1994), MS thesis, University of Illinois, Urbana.Google Scholar
  10. 10.
    Tejayadi, S. and Cheryan, M. (1995) Appl. Microbiol. Biotechnol. 43, 242–248.CrossRefGoogle Scholar
  11. 11.
    A. Anon. (1994) Chem. Marketing Reporter. 245, 28–36.Google Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • In Soo Han
    • 1
  • Munir Cheryan
    • 1
  1. 1.Agricultural Bioprocess LaboratoryUniversity of IllinoisUrbanaUSA

Personalised recommendations