Abstract
An ammonia-based pretreatment method termed ammonia recycled percolation (ARP) was developed for pretreating herbaceous biomass like corn cobs/stover mixture (CCSM) and switchgrass. The process involves treatment of biomass with aqueous ammonia through a percolation reactor (packed-bed, flow-through type). The effects temperature, reaction time, and ammonia concentration were studied. The extent of delignification in the ARP process was in the range of 60-80% for CCSM and 65-85% for switchgrass. The ARP process solubilized significant amounts of the hemicellulose fraction into the pretreatment effluent, yet left most of the glucan fraction intact. The experimental data on CCSM and switchgrass indicate that the ARP is a highly effective pretreatment method. Near-complete conversion of cellulose to glucose was obtained by enzymatic hydrolysis of ARP-treated solid samples of CCSM, whereas conversion was slightly lower for switchgrass. The rate of enzymatic hydrolysis of ARP-treated samples was substantially higher than that of a-cellulose. The ARP effluents were evaluated for fermentability/toxicity by the xylose-fermenting yeastPichia stipitis(NRRL Y-11545). The adaptability of ARP-treated solid samples to simultaneous saccharification and fermentation (SSF) was tested for ethanol production using cellulase enzyme and the yeast, Saccharomyces cerevisiae (NREL, D5A).
Author to whom all correspondence and reprint requests should be addressed.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Cahela, D. R., Lee, Y. Y., and Chambers, R. P. (1983) Biotechnol. Bioeng. 25, 3–18.
McGinnis, G. D., Wilson, W. W., Mullen, C. E. (1983) Ind. Eng. Chem. Prod. Res. Dev. 22, 352–357.
Gould, J. M. (1984) Biotechnol. Bioeng. 27, 225–231.
Vallander, L. and Eriksson, K.-E. (1985) Biotechnol. Bioeng. 27, 650–659.
Ropars, M., Marchai, R., Pourquie, J., and Vandecasteele, J. P. (1992) Bioresource Technol. 42, 197–204.
Holtzapple, M. T., Lundeen, J. E., Sturgis, R., Lewis, J. E., and Dale, B. E. (1992) Appl. Biochem. Biotechol. 34/35, 5–21.
Torget, R. and Hsu, T.-A. (1994) Appl. Biochem. Biotechnol. 45/46, 5–22.
Detroy, R. W., Lindenfelser, L. A., Sommer, S., and Orton, W. L. (1981) Biotechnol. Bioeng. 23, 1527.
Averinos, G. C. and Wang, D. I. C. (1983) Biotechnol. Bioeng. 25, 67.
Yoon, H. H., Wu, Z. W., and Lee, Y. Y. (1995) Appl. Biochem. Biotechnol. 51/52, 5–19.
Turhollow, A. F. (1991) Bioresource Technol. 36, 247–252.
Ehrman, T. and Magill, K. (1992), NREL-CAT Standard Procedure No. 001–003, Golden, CO.
Philippidis, G. P., Smith, T. K., and Schmidt, S. L. (1993), NREL-CAT Standard Procedure No. 008, Golden, CO.
Kurtzman, C. P. (1994) P. stipitis-Based Pretreatment Liquor Fermentability/Toxicity Test Protocol (USDA), Peoria, IL.
Dale, B. E., Henk, L. L., and Striang, M. (1985) Dev. Ind. Microb. 26, 223–233.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media New York
About this chapter
Cite this chapter
Iyer, P.V., Wu, ZW., Kim, S.B., Lee, Y.Y. (1996). Ammonia Recycled Percolation Process for Pretreatment of Herbaceous Biomass. In: Wyman, C.E., Davison, B.H. (eds) Seventeenth Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium, vol 57/58. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0223-3_11
Download citation
DOI: https://doi.org/10.1007/978-1-4612-0223-3_11
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-6669-3
Online ISBN: 978-1-4612-0223-3
eBook Packages: Springer Book Archive