Abstract
Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to “fingerprint” analysis by high-resolution 2-D gel electro-phoresis. Forty-five spots from 11 × 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of compo-nent enzymes from the T. reesei cellulase system acting on complex sub-strates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggestfollow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.
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Vinzant, T.B. et al. (2001). Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation. In: Davison, B.H., McMillan, J., Finkelstein, M. (eds) Twenty-Second Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0217-2_8
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DOI: https://doi.org/10.1007/978-1-4612-0217-2_8
Publisher Name: Humana Press, Totowa, NJ
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