Abstract
The dissemination of catabolic plasmids was compared to bioaugmentation by strain inoculation in microcosm experiments. When Rhodococcus erythropolis strain T902, bearing a plasmid with trichloroethene and isopropylbenzene degradation pathways, was used as the inoculum, no transconjugant was isolated but the strain remained in the soil. This plasmid had a narrow host range. Pseudomonas putida strain C8S3 was used as the inoculum in a second approach. It bore a broad host range conjugative plasmid harboring a natural transposon, RP4::Tn4371, responsible for biphenyl and 4-chlorobiphenyl degradation pathways. The inoculating population slowly decreased from its original level (106 colony-forming units [CFU]/g of dry soil) to approx 3 x 102 CFU/g of dry soil after 3 wk. Transconjugant populations degrading biphenyl appeared in constant humidity soil (up to 2 x 103 CFU/g) and desiccating soil (up to 104 CFU/g). The feasibility of plasmid dissemination as a bioaugmentation technique was demonstrated in desiccating soils. The écologie significance of desiccation in bioaugmentation was demonstrated: it upset the microbial ecology and the development of transconjugants.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Jasper, D. (1994), Aust. J. Soil Res. 32, 1301–1319.
Rittmann, B. and Whiteman, R. (1994), Water Qual. Int. 1, 12–16.
Möller, J., Steckel, T., Wedebye, E., and Werstermann, P. (1995), Bull. Environ. Contam. Toxicol. 54, 913–918.
Vogel, T. (1996), Curr. Ovin. Biotechnol. 7, 311–316.
Weekers, F., Jacques, P., Springael, D., Mergeay, M., Diels, L., and Thonart, P. (1998), Appl. Biochem. Biotechnol. 70–72, 311–322.
Weekers, F., Jacques, P., Springael, D., Mergeay, M., Diels, L., and Thonart, P. (1996), Med. Vac. Landbouww. Univ. Gent 61/4b, 2161–2164.
Potts, M. (1994), Microbiol. Rev. 58, 755–805.
Weekers, F., Jacques, P., Springael, D., Mergeay, M., Diels, L., and Thonart, P. (1999), Appl. Biochem. Biotechnol. 77–79, 251–266.
Sayler, G., Hooper, S., Layton, A., and King, J. (1990), in Microbial Ecology, Fietcher, M., ed., Springer-Verlag, New York.
Brokamp, A. and Schmidt, F. (1991), Curr. Microbiol. 22, 299–306.
Daane, J., Molina, J., Berry, E., and Sadowski, M. (1996), Appl. Environ. Microbiol. 62, 515–521.
De Rore, H., Demolder, K., De Wilde, K., Top, E., Houwen, F., and Verstraete, W. (1994), FEMS Microbiol. Ecol. 15, 71–78.
Di Giovanni, G., Neilson, J., Pepper, I., and Sinclair, N. (1996), Appl. Environ. Microbiol. 62, 2521–2526.
Top, E., Van Daele, P., De Saeyer, N., and Forney, L. (1998), Antonie Van Leeuwenhoek 73, 87–94.
Dong, Q., Springael, D., Schoters, J., Nuyts, G., Mergeay, M., and Diels, L. (1998), Water Sci. Technol. 37, 465–468.
Margesin, R. and Schinner, F. (1997), J. Bas. Microbiol. 37, 217–227.
Dabrock, B., Kesseler, M., Averhoff, B., and Gottschalk, G. (1994), Appl. Environ. Microbiol. 60, 853–860.
Springael, D., Kreps, S., and Mergeay, M. (1993), J. Bacterial. 175, 1674–1681.
Weekers, F., Jacques, P., Springael, D., Mergeay, M., Diels, L., and Thonart, P. (2000), in Focus on Biotechnology, Hofman, M. and Anne, J., eds., Kluwer Academic, Amsterdam.
Galli, E., Barbieri, P., and Bestteti, G. (1992), in Pseudomonas: Molecular Biology and Biotechnology, Galli, E., Silver, S., and Withold, B., eds., American Society for Micro-biology, Washington, DC.
Kesseler, M., Dabbs, E., Averhoff, B., and Gottschalk, G. (1996), Microbiology 142, 3241–3251.
Paul, E. and Clark, F. (1988), Soil Microbiology and Biochemistry, Academic Press, London.
Mergeay, M. and Springael, D. (1996), in Bioremediation Protocols, Sheehan, D., ed., Humana Press, Totowa, NJ.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this chapter
Cite this chapter
Weekers, F., Rodriguez, C., Jacques, P., Mergeay, M., Thonart, P. (2001). Dissemination of Catabolic Plasmids among Desiccation-Tolerant Bacteria in Soil Microcosms. In: Davison, B.H., McMillan, J., Finkelstein, M. (eds) Twenty-Second Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0217-2_20
Download citation
DOI: https://doi.org/10.1007/978-1-4612-0217-2_20
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-4612-6667-9
Online ISBN: 978-1-4612-0217-2
eBook Packages: Springer Book Archive