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Twenty-Second Symposium on Biotechnology for Fuels and Chemicals pp 147–154Cite as

Isolation of Magnetospirillum magneticum AMB-1 Mutants Defective in Bacterial Magnetic Particle Synthesis by Transposon Mutagenesis

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Abstract

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10−7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analy- sis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

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References

  1. Schuler, D. and Frankel, R. B. (1999), Appl. Microbiol. Biotechnol. 52, 464–473.

    Article  CAS  Google Scholar 

  2. Blackemore, R. P., Maratea, D., and Wolfe, R. S. (1979), J. Bacteriol. 140, 720–729.

    Google Scholar 

  3. Matsunaga, T., Sakaguchi, T., and Tadokoro, F. (1991), Appl. Microbiol. Biotechnol. 35, 651–655.

    Article  CAS  Google Scholar 

  4. Matsunaga, T., Nakamura, C., Burgess, J. G., and Sode, K. (1992), J. Bacteriol. 174, 2748–2753.

    CAS  Google Scholar 

  5. DeBruijn, F. J. and Lupski, J. R. (1984), Gene 27, 131–149.

    Article  CAS  Google Scholar 

  6. Simon, R., Preifer, U., and Puhler, A. (1983), Bio/Technology 1, 784–791.

    Article  CAS  Google Scholar 

  7. Berg, D. E. (1989), in Mobile DNA, Berg, D. E and Howe, M. M., eds., American Society for Microbiology, Washington, DC, pp. 185–210.

    Google Scholar 

  8. Herrero, M., deLorenzo, V., and Timmis, K. N. (1990), J. Bacteriol. 172, 6557–6567.

    CAS  Google Scholar 

  9. Holtwick, R., Meinhardt, F., and Keweloh, H. (1997), Appl. Environ. Microbiol. 63, 4292–4297.

    CAS  Google Scholar 

  10. Jiang, Z. Y., Rushing, B. G., Bai, Y., Gest, H., and Bauer, C. E. (1998), J. Bacteriol. 180, 1248–1255.

    CAS  Google Scholar 

  11. DeLorenzo, V., Herrero, M., Martinko, J. M., and Parker, J. (1990), J. Bacteriol. 172, 6568–6572.

    CAS  Google Scholar 

  12. Martin, V. J. J. and Mohn, W. W. (1999), J. Microbiol. Methods 35, 163–166.

    Article  CAS  Google Scholar 

  13. Nakamura, C., Burgess, J. G., Sode, K., and Matsunaga, T. (1995), J. Biol. Chetn. 270, 28,392–28,396.

    CAS  Google Scholar 

  14. Wilson, K. (1994), in Current Protocols in Molecular Biology, vol. 1, Ausubel, F. M., Brent, R., and Kingston, R. E., eds., John Wiley & Sons, New York, pp. 2.4.1–2.4.5.

    Google Scholar 

  15. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York.

    Google Scholar 

  16. Klarskov, K., Van Driessche, G., Barkers, K., Dumortier, C., Meyer, T. E., Tollin, G., Cusanovich, M. A., and Beeumen, J. J. V. (1998), Biochemistry 37, 5995–6002.

    Article  CAS  Google Scholar 

  17. Wilson, R., Ainscough, R., Anderso, K., et al. (1994), Nature 368, 32–38.

    Article  CAS  Google Scholar 

  18. Freiberg, C., Fellay, R., Bairoch, A., Broughton, W. J., Rosenthal, A., and Parret, X. (1997), Nature 387, 394–401.

    Article  CAS  Google Scholar 

  19. Kaneko, T., Sato, S., Kotani, H., et al. (1996), DNA Res. 3, 109–136.

    Article  CAS  Google Scholar 

  20. Tietze, E. (1998), Plasmid 39, 165–168.

    Article  CAS  Google Scholar 

  21. Sherman, D. H., Malpartida, F., Bibb, M. J., Kieser, H. M., and Hopwood, D. A. (1989), EMBO J. 8, 2717–2725

    CAS  Google Scholar 

  22. Ramakrishnan, G., Zhao, J. L., and Newton, A. (1994), J. Bacteriol. 176, 7587–7600.

    CAS  Google Scholar 

  23. Deckart, G. and Warren, P. V. (1998), Nature 392, 353–358.

    Article  Google Scholar 

  24. Davison, J., Brunei, F., Phanopoulos, A., Prozzi, D., and Terpstra, P. (1992), Gene 114, 19–24.

    Article  CAS  Google Scholar 

  25. Ooi, J., Yatnik, V., Immanuel, D., Gordon, M., Moskow, J. J., Buchberg, A. M., and Margolis, B. (1995), Oncogene 10, 1621–1630.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Naka-Cho, Koganei, Tokyo, 184-8588, Japan

    Aris Tri Wahyudi, Haruko Takeyama & Tadashi Matsunaga

Authors
  1. Aris Tri Wahyudi
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  2. Haruko Takeyama
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  3. Tadashi Matsunaga
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Editor information

Editors and Affiliations

  1. Oak Ridge National Laboratory, USA

    Brian H. Davison

  2. National Renewable Energy Laboratory, USA

    James McMillan  & Mark Finkelstein  & 

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© 2001 Springer Science+Business Media New York

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Wahyudi, A.T., Takeyama, H., Matsunaga, T. (2001). Isolation of Magnetospirillum magneticum AMB-1 Mutants Defective in Bacterial Magnetic Particle Synthesis by Transposon Mutagenesis. In: Davison, B.H., McMillan, J., Finkelstein, M. (eds) Twenty-Second Symposium on Biotechnology for Fuels and Chemicals. ABAB Symposium. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0217-2_12

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  • DOI: https://doi.org/10.1007/978-1-4612-0217-2_12

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6667-9

  • Online ISBN: 978-1-4612-0217-2

  • eBook Packages: Springer Book Archive

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