Sphaerotilus natans Isolated from Activated Sludge and Its Production of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)

  • Kang Liu
  • Hong Chua
  • Wai-hung Lo
  • Hugh Lawford
  • Peter Hoi-Fu Yu
Part of the Applied Biochemistry and Biotechnology book series (ABAB)


Sphaerotilus natans is a sheathed bacterium existing in the activated sludge of wastewater treatment plants. It is one of the filamentous bacteria causing the bulking and foaming difficulties of activated sludge. Isolating the strain and culturing it in an axenic environment could not only provide the metabolic knowledge of the strains that would be useful in the development of wastewater treatment methods, but also could enable us to gain an understanding of the mechanism by which poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (poly[3-HB-co-3-HV]) is produced by this strain. This article reports the screening and isolation of the strain from the activated sludge using the Nile blue staining method together with Fourier transform infrared analysis. We investigated the ability of the selected strain to produce poly(3-HB-co-3-HV) copolymer using glucose and peptone, or by adding valeric acid or sodium propionate as precursor. Proper precursor feeding could dramatically enhance its 3HV content in the copolymer P(3HB-co-3HV). By controlling the different feeding times in fed-batch fermentation, different desired copolymers were obtained with 15, 40, and 70% 3HV mole fraction of the copolymer. Polymer properties were analyzed by gas chromatography, differential scanning calorimetry, thermo-gravimetry, and nuclear magnetic resonance analysis.

Index Entries

Activated sludge copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) polymer properties Sphaerotilus nutans 


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  1. 1.
    Bluhm, T. L., Hamer, G. K., Marchessault, R. H., Fyfe, C. A., and Veregin, R. P. (1986), Macromolecules 19, 2871–2876.CrossRefGoogle Scholar
  2. 2.
    Bloemnergen, S., Holden, D. A, Harmer, G. K, Bluhm, T. L, and Marchessault, R. H. (1986), Macromolecules 19, 2865–2871.CrossRefGoogle Scholar
  3. 3.
    Mergaert, J., Webb, A., Anderson, C., Wouters, A., and Swing, J. (1993), Appl. Environ. Microbiol. 59, 3233–3238.PubMedGoogle Scholar
  4. 4.
    Cyras, V. P., Galego, F. N., and Vazquez, A. (1999), Polym. Int. 48, 705.CrossRefGoogle Scholar
  5. 5.
    Galego, N., Rozsa, C., Sanchez, R., Fung, J., Vazquez, A., and Tomas, J.S. (2000), Polymer Testing 19, 485–492.CrossRefGoogle Scholar
  6. 6.
    Dondero, N. C. (1961), Adv. Appl. Microbiol. 3, 77–107.PubMedCrossRefGoogle Scholar
  7. 7.
    Veen, W. L., Mulder, E. G., and Deinema, M. H. (1978), Microbiol. Rev. 42, 329–356.PubMedGoogle Scholar
  8. 8.
    Takeda, M., Matsuoka, H., Ban, H., Ohashi, Y., Hikuma, M., and Koizumi, Ji. (1995), Appl. Microbiol. Biotechnol. 44, 37–42.CrossRefGoogle Scholar
  9. 9.
    Hu, W. F., Chua, H., and Yu, H. F. (1997), Biotechnol. Lett. 7, 695–698.CrossRefGoogle Scholar
  10. 10.
    Chua, H., Yu, H. F., and Lo, W. (1998), Appl. Biochem. Biotechnol. 70–72, 929–935.PubMedCrossRefGoogle Scholar
  11. 11.
    Chua, H., Yu, H. F., and Ma, C. K. (1999), Appl. Biochem. Biotechnol. 77–79, 389–399.PubMedCrossRefGoogle Scholar
  12. 12.
    Shimizu, H., Kozaki, Y., Kodama, H., and Shioya, S. (1999), Biotech. Bioeng. 62, 518–552.CrossRefGoogle Scholar
  13. 13.
    Kitamura, S. and Doi, Y. (1994), Biotech. Tech. 8, 345–350.CrossRefGoogle Scholar
  14. 14.
    Hong, K., Leung, Y. C., Kwok, S. Y., Law, K. H., Lo, W. H., Chua, H., and Yu, P. H. (2000), Appl. Biochem. Biotechnol. 84, 381–390.PubMedCrossRefGoogle Scholar
  15. 15.
    Yu, H. P., Chua, H., Huang, A. L., Lo, W., and Chen, G. Q. (1998), Appl. Biochem. Biotechnol. 70–72, 603–614.PubMedCrossRefGoogle Scholar
  16. 16.
    Yu, H. F., Chua, H., Huang, A. L., Lo, W. H., and Ho, K. P. (1999), Water Sci. Technol. 40, 365–370.Google Scholar
  17. 17.
    Madison, L. L. and Huisman, G. W. (1999), Microbiol. Mol. Biol. Rev. 63, 21–53.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Kang Liu
    • 1
  • Hong Chua
    • 2
  • Wai-hung Lo
    • 1
  • Hugh Lawford
    • 3
  • Peter Hoi-Fu Yu
    • 1
  1. 1.Department of Applied Biology and Chemical TechnologyHong Kong
  2. 2.Department of Civil and Structural EngineeringHong Kong Polytechnic UniversityHong KongChina
  3. 3.Department of BiochemistryUniversity of TorontoTorontoCanada

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