SO2-Catalyzed Steam Explosion of Corn Fiber for Ethanol Production

  • Renata Bura
  • Shawn D. Mansfield
  • John N. Saddler
  • Rodney J. Bothast
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

Corn fiber, a by-product of the corn wet-milling industry, represents a renewable resource that is readily available in significant quantities and could potentially serve as a low-cost feedstock for the production of fuel-grade alcohol. In this study, we used a batch reactor to steam explode corn fiber at various degrees of severity to evaluate the potential of using this feedstock in the bioconversion process. The results indicated that maximum sugar yields (soluble and following enzymatic hydrolysis) were recovered from corn fiber that was pretreated at 190°C for 5 min with 6% SO2. Sequential SO2-catalyzed steam explosion and enzymatic hydrolysis resulted in very high conversion (81%) of all polysaccharides in the corn fiber to monomeric sugars. Subsequently, Saccharomyces cerevisiae was able to convert the resultant corn fiber hydrolysates to ethanol very efficiently, yielding 90–96% of theoretical conversion during the fermentation process.

Index Entries

Corn fiber steam pretreatment enzymatic hydrolysis fermentation ethanol 

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References

  1. 1.
    Grohmann, K., Torget, R., and Himmel, M. (1985), Biotechnol. Bioeng. Symp. 15, 59–80.Google Scholar
  2. 2.
    Grohmann, K. and Bothast, R. J. (1996), Process Biochem. 32, 405–415.CrossRefGoogle Scholar
  3. 3.
    Excoffier, G., Toussaint, B., and Vignon, M. R. (1991), Biotechnol. Bioeng. 38, 1308–1317.PubMedCrossRefGoogle Scholar
  4. 4.
    Gregg, D. J., Boussaid, A., and Saddler, J. N. (1998), Bioresour. Technol. 63, 7–12.CrossRefGoogle Scholar
  5. 5.
    O’Connor, D. (2000), Liquid Fuels from Biomass: North American Impact on Non-technical Barriers on Implementation, Report IEA Bioenergy Task 27, Delta, Canada, pp. 34–46.Google Scholar
  6. 6.
    Anderson, R. A. and Watson, S. A. (1982), in Handbook of Processing and Utilization in Agriculture, vol. 2, Wolff, I. A. ed., CRC, Boca Raton, FL, pp. 31–61.Google Scholar
  7. 7.
    Osborn, D. and Chen, L. F. (1984), Starch/Stärke. 36, 393–395.CrossRefGoogle Scholar
  8. 8.
    Beall, D. S., Ingram, L. O., Ben-Bassat, A., Doran, J. B., Fowler, D. E., Hall, R. G., and Wood, B. E. (1992),Biotechnol. Lett. 14, 857–862.CrossRefGoogle Scholar
  9. 9.
    Moniruzzaman, M., Dale, B. E., Hespell, R. B., and Bothast, R. J. (1997), Appl. Biochem. Biotechnol. 67, 113–126.CrossRefGoogle Scholar
  10. 10.
    Clark, T. A. and Mackie, K. L. (1987), J. Wood Chem. Technol. 7, 373–403.CrossRefGoogle Scholar
  11. 11.
    Schwald, W., Smaridge, T., Chan, M., Breuil, C., and Saddler, J. N. (1987), in Enzyme Systems for Lignocellulose Degradation, Coughlan, M.P. ed., Elsevier, NY, pp. 231–242.Google Scholar
  12. 12.
    Clark, T. A., Mackie, K. L., Dare, P. H., and McDonald, A. G. (1989), J. Wood Chem. Technol. 9, 135–166.CrossRefGoogle Scholar
  13. 13.
    Carrasco, J. E., Ma, C., Saiz, A., Navarro, P., Soriano, P., Saez, F., and Martinez, J. M. (1994), Appl. Biochem. Biotechnol. 45,46, 23–34.CrossRefGoogle Scholar
  14. 14.
    Boussaid, A., Esteghlalian, A. R., Gregg, D. J., Lee, K. H., and Saddler, J. N. (2000), Appl. Biochem. Biotechnol. 84–86, 693–705.PubMedCrossRefGoogle Scholar
  15. 15.
    Brownell, H. H., Yu, E. K. C., and Saddler, J. N. (1986), Biotechnol. Bioeng. 27, 792–801.CrossRefGoogle Scholar
  16. 16.
    Mackie, K. L., Brownell, H. H., West, K. L., and Saddler, J. N. (1985), J. Wood Chem. Technol. 5, 405–425.CrossRefGoogle Scholar
  17. 17.
    Chum, H. L., Johnson, D. K., Black, S., Baker, J., Grohmann, K., Sarkanen, K. V., Wallace, K., and Schroeder, H. A. (1988), Biotechnol. Bioeng. 31, 643–649.PubMedCrossRefGoogle Scholar
  18. 18.
    Boussaid, A. and Saddler, J. N. (1999), Enzyme Microb. Technol. 24, 138–143.XCrossRefGoogle Scholar
  19. 19.
    Boussaid, A., Robinson, J., Cai, Y. J., Gregg, D. J., and Saddler, J. N. (1999), Biotechnol. Bioeng. 64, 284–289.PubMedCrossRefGoogle Scholar
  20. 20.
    Shevchenko, S. M., Beatson, R. P., and Saddler, J. N. (1999), Appl. Biochem. Biotechnol. 77–79, 867–876.PubMedCrossRefGoogle Scholar
  21. 21.
    Michalowicz, G., Toussaint, B., and Vignon, M. R. (1991), Holzforschung 45, 175–179.CrossRefGoogle Scholar
  22. 22.
    Overend, R. P. and Chornet, E. (1987), Phil. Trans. R. Soc. Lond. 321, 523–536.CrossRefGoogle Scholar
  23. 23.
    Brownell, H. H., Schwald, W., Smaridge, T., and Saddler, J.N. (1988), in Proceedings of the IEA Workshop on Bioconversion of Lignocellulosics, Saddler, J. N., Stevens, D., and Hages, D., eds., Forintek Canada, Ottawa, Ontario, pp. A–1.Google Scholar
  24. 24.
    TAPPI, Technical Association of the Pulp and Paper Industry (1998), TAPPI Standard Methods, T-222 om-98, TAPPI Press, Atlanta, GA.Google Scholar
  25. 25.
    Ghose, T. K. (1987), Pure Appl. Chem. 59, 257–268.CrossRefGoogle Scholar
  26. 26.
    Olsson, L., Hahn-Hägerdal, B., and Zacchi, G. (1995), Biotechnol. Bioeng. 45, 356–365.PubMedCrossRefGoogle Scholar
  27. 27.
    Whistler, R. L. (1993), in Industrial Gums: Polysaccharides and Their Derivatives, 3rd ed., Whistler, R. L. and BeMiller, J. N., eds., Academic, San Diego, CA, pp. 294–308.Google Scholar
  28. 28.
    BeMiller, J. N. (1967), Adv. Carbohydr. Chem., 22, 25–107.CrossRefGoogle Scholar
  29. 29.
    Sjolander, N. O., Langlykke, A. F., and Peterson, W. F. (1938), Ind. Eng. Chem. 30, 1251–1255.CrossRefGoogle Scholar
  30. 30.
    Dien, B. S., Iten, L. B., and Bothast, R. J. (1999), J. Ind. Microb. Biotechnol. 22, 575–581.CrossRefGoogle Scholar
  31. 31.
    Parajó, J. C., Dominguez, H., and Dominquez, J. M. (1997), Enzyme Microb. Technol. 21, 18–24.CrossRefGoogle Scholar
  32. 32.
    Larsson, S., Reimann, A., Nilvebrant, N. O. and Jönsson, L. J. (1999), Appl. Biochem. Biotechnol. 77–79, 91–103.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • Renata Bura
    • 1
  • Shawn D. Mansfield
    • 2
  • John N. Saddler
    • 1
  • Rodney J. Bothast
    • 2
  1. 1.Forest Products Biotechnology, Department of Wood ScienceUniversity of British ColumbiaVancouverCanada
  2. 2.Fermentation BiochemistryNational Center for Agricultural Utilization Research, USDA, ARSPeoriaUSA

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