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Isolation and Characterization of Thermophilic Benzothiophene-Degrading Mycobacterium sp.

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Biotechnology for Fuels and Chemicals

Abstract

A bacterial strain, SWU-4, capable of using benzothiophene (BT) as a sole carbon and energy source was isolated from a petroleum-contaminated site in Thailand and identified by 16S rRNA gene sequence analysis to be in the genus of Mycobacterium. The strain was Gram-positive, nonspore former, and grew at 50° C. Colonies of the strain on nutrient agar were rod-shaped, smooth with a convex surface, slightly mucoid, and yellow pigmented. The thermophilic Mycobacterium sp. strain SWU-4 rapidly degraded 2% (w/v) BT at 50°C. Interestingly, this strain was able to degrade a wide variety of organosulfur compounds including thiophene, bromo(oc)thiophene, and 3-methylthiophene in liquid minimum medium at 50°C, which will be beneficial for industrial applications.

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Watanapokasin, Y., Nuchfoang, S., Nilwarangkoon, S., Sarangbin, S., kakizono, T. (2002). Isolation and Characterization of Thermophilic Benzothiophene-Degrading Mycobacterium sp.. In: Finkelstein, M., McMillan, J.D., Davison, B.H. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0119-9_24

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  • DOI: https://doi.org/10.1007/978-1-4612-0119-9_24

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6621-1

  • Online ISBN: 978-1-4612-0119-9

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