Optimizing Ammonia Processing Conditions to Enhance Susceptibility of Legumes to Fiber Hydrolysis
An ammonia process was applied at several ammonia loadings, moisture contents, temperatures, and dwell times. A cellulase loading of 5 FPU/g dry matter and a 24 h incubation time were used to produce the sugars, which were measured as reducing sugars and by HPLC. Optimal processing conditions caused a 76% of theoretical yield (2.9-fold above untreated). Cellulose and hemicellulose conversions were 68 and 85% (vs 38 and 34% in untreated, respectively). The short hydrolysis time and relatively low enzyme loading suggests great potential to produce sugars from alfalfa.
Index EntriesAmmonia alfalfa enzymatic hydrolysis sugars
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- 1.Sulbarán-de-Ferrer, B., Ferrer, A., Byers, F. M., Dale, B. E. and Aristiguieta, M. (1997), Arch. Latinamer. Prod. Anim. 5(Suppl. 1), 112–114.Google Scholar
- 4.Goering, H. K. and P. J. Van Soest. (1970), Handbook No. 379. ARS-USDA, Washington, DC.Google Scholar
- 7.SAS (1985), SAS Institute Inc., Cary, NC.Google Scholar
- 8.Ferrer, A., Byers, F. M., Sulbarán-de-Ferrer, B., and Dale, B. E. (2002), Anim. Feed Sci. Technol. (submitted).Google Scholar
- 15.Bracho, R., Colina, A., Sulbaran-de-Ferrer, B., Ferrer, A., Parra, P., Peters, J., and Rumbos, C. (2001), V Congreso Venezolano de Química, Maracaibo, Venezuela, pp. 637–640.Google Scholar
- 17.Zabala, I, Ferrer, A., Ledesma, A., and Aiello, C. (1994), in Advances in Bioprocess Engineering, Galindo, E. and Ramirez, O.T., eds., Kluwer Academic Publishers, London, pp. 455–460.Google Scholar