Optimizing Ammonia Processing Conditions to Enhance Susceptibility of Legumes to Fiber Hydrolysis

  • A. Ferrer
  • F. M. Byers
  • B. Sulbarán-De-Ferrer
  • B. E. Dale
  • C. Aiello
Part of the Applied Biochemistry and Biotechnology book series (ABAB)


An ammonia process was applied at several ammonia loadings, moisture contents, temperatures, and dwell times. A cellulase loading of 5 FPU/g dry matter and a 24 h incubation time were used to produce the sugars, which were measured as reducing sugars and by HPLC. Optimal processing conditions caused a 76% of theoretical yield (2.9-fold above untreated). Cellulose and hemicellulose conversions were 68 and 85% (vs 38 and 34% in untreated, respectively). The short hydrolysis time and relatively low enzyme loading suggests great potential to produce sugars from alfalfa.

Index Entries

Ammonia alfalfa enzymatic hydrolysis sugars 


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  1. 1.
    Sulbarán-de-Ferrer, B., Ferrer, A., Byers, F. M., Dale, B. E. and Aristiguieta, M. (1997), Arch. Latinamer. Prod. Anim. 5(Suppl. 1), 112–114.Google Scholar
  2. 2.
    Ferrer, A., Byers, F. M, Sulbaran-de-Ferrer, B., Dale, B. E. and Aiello, C. (2000), Appl. Biochem. Biotechnol. 84–86, 163–179.PubMedCrossRefGoogle Scholar
  3. 3.
    Kephart, K. D., D. R. Buxton, and Hill Jr., R. R. (1990), Crop. Sci. 30, 207–212.CrossRefGoogle Scholar
  4. 4.
    Goering, H. K. and P. J. Van Soest. (1970), Handbook No. 379. ARS-USDA, Washington, DC.Google Scholar
  5. 5.
    Ghose, T.K. (1987), Pure Appl. Chem. 59, 257–268.CrossRefGoogle Scholar
  6. 6.
    Miller, G.L. (1959), Anal. Chem. 31, 426–428.CrossRefGoogle Scholar
  7. 7.
    SAS (1985), SAS Institute Inc., Cary, NC.Google Scholar
  8. 8.
    Ferrer, A., Byers, F. M., Sulbarán-de-Ferrer, B., and Dale, B. E. (2002), Anim. Feed Sci. Technol. (submitted).Google Scholar
  9. 9.
    Ben-Ghedalia, D. and Miron, J. (1984), J. Nutr. 114, 880–887.PubMedGoogle Scholar
  10. 10.
    Lechtenberg, V. L., Holt, D. A. and Youngberg, H. W. (1971), Agron. J. 63, 719–724.CrossRefGoogle Scholar
  11. 11.
    Mandels, M., Hontz, L. and Nystrom, J. (1974), Biotechnol. Bioeng. 16, 1471–1493.CrossRefGoogle Scholar
  12. 12.
    Collins, M. (1986), Agron. J. 78, 1018–1022.CrossRefGoogle Scholar
  13. 13.
    De la Rosa, L. B., Reshamwala, S., Latimer, V. M., Shawky, B. T., Dale, B. E., and Stuart, E. D. (1994) Appl. Biochem. Biotechnol. 45/46, 483–497.CrossRefGoogle Scholar
  14. 14.
    Holtzapple, M. T., Ripley, E. P., and Nikolaou, M. (1994), Biotechnol. Bioeng. 44, 1122–1131.PubMedCrossRefGoogle Scholar
  15. 15.
    Bracho, R., Colina, A., Sulbaran-de-Ferrer, B., Ferrer, A., Parra, P., Peters, J., and Rumbos, C. (2001), V Congreso Venezolano de Química, Maracaibo, Venezuela, pp. 637–640.Google Scholar
  16. 16.
    Ramos, L. P., Breuil, C, and Saddler, J. N. (1993), Enzyme Microb. Technol. 15, 19–25.CrossRefGoogle Scholar
  17. 17.
    Zabala, I, Ferrer, A., Ledesma, A., and Aiello, C. (1994), in Advances in Bioprocess Engineering, Galindo, E. and Ramirez, O.T., eds., Kluwer Academic Publishers, London, pp. 455–460.Google Scholar
  18. 18.
    Aiello, C, Ferrer, A., and Ledesma, A. (1996), Bioresour. Technol. 57, 13–18.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • A. Ferrer
    • 1
  • F. M. Byers
    • 2
  • B. Sulbarán-De-Ferrer
    • 1
  • B. E. Dale
    • 3
  • C. Aiello
    • 4
  1. 1.Department of Chemistry, Science FacultyUniversity of ZuliaMaracaiboVenezuela
  2. 2.Department of Animal ScienceTexas A&M UniversityCollege StationUSA
  3. 3.Department of Chemical EngineeringMichigan State UniversityEast LansingUSA
  4. 4.Engineering FacultyUniversity of ZuliaMaracaiboVenezuela

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