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Optimizing Ammonia Processing Conditions to Enhance Susceptibility of Legumes to Fiber Hydrolysis

Alfalfa
  • A. Ferrer
  • F. M. Byers
  • B. Sulbarán-De-Ferrer
  • B. E. Dale
  • C. Aiello
Chapter
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

An ammonia process was applied at several ammonia loadings, moisture contents, temperatures, and dwell times. A cellulase loading of 5 FPU/g dry matter and a 24 h incubation time were used to produce the sugars, which were measured as reducing sugars and by HPLC. Optimal processing conditions caused a 76% of theoretical yield (2.9-fold above untreated). Cellulose and hemicellulose conversions were 68 and 85% (vs 38 and 34% in untreated, respectively). The short hydrolysis time and relatively low enzyme loading suggests great potential to produce sugars from alfalfa.

Index Entries

Ammonia alfalfa enzymatic hydrolysis sugars 

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • A. Ferrer
    • 1
  • F. M. Byers
    • 2
  • B. Sulbarán-De-Ferrer
    • 1
  • B. E. Dale
    • 3
  • C. Aiello
    • 4
  1. 1.Department of Chemistry, Science FacultyUniversity of ZuliaMaracaiboVenezuela
  2. 2.Department of Animal ScienceTexas A&M UniversityCollege StationUSA
  3. 3.Department of Chemical EngineeringMichigan State UniversityEast LansingUSA
  4. 4.Engineering FacultyUniversity of ZuliaMaracaiboVenezuela

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