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Active Nuclear Shuffling System Using a Swollen Conidium of Trichoderma reesei

  • Hideo Toyama
  • Makiko Yano
  • Akane Gisushi
  • Takeshi Hotta
  • Nobuo Toyama
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

Cellulase hyperproducers of Trichoderma reesei can be constructed using autopolyploidization and haploidization techniques. To increase the efficiency of this method, the active nuclear shuffling system in a swollen conidium was effective. A dried mature green conidium of a model strain, T. reesei QM6a (IFO 31326), was swollen to make room for a larger autopolyploid nucleus. After colchicine treatment, a larger autopolyploid nucleus was produced in such a swollen conidium. Benomyl treatment of swollen conidia generated multiple smaller nuclei from one larger autopolyploid nucleus. Those smaller nuclei were transported through conidia to mycelia after germination. This system could contribute to increasing the efficiency of genetic shuffling.

Index Entries

Trichoderma reesei cellulose colchicines cellulose benomyl 

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Hideo Toyama
    • 1
  • Makiko Yano
    • 1
  • Akane Gisushi
    • 1
  • Takeshi Hotta
    • 1
  • Nobuo Toyama
    • 1
  1. 1.Department of Food Science and Technology, Faculty of HorticultureMinamikyushu UniversityMiyazakiJapan

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