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Xylanase Production by Trichoderma reesei Rut C-30 on Rice Straw

  • Alejandro Colina
  • Betzabé Sulbarán-de-Ferrer
  • Cateryna Aiello
  • Alexis Ferrer
Part of the Applied Biochemistry and Biotechnology book series (ABAB)

Abstract

Xylanase production of Trichoderma reesei Rut C-30 was examined at different initial pH values (4.8, 5.9, and 7.0) on rice straw in shake flasks, and in a fermentor, for the best pH condition. Enzyme performance was tested on ammonia-treated dwarf elephant grass. The maximum xylanase activities, 92 and 122 IU/mL, were obtained at pH 4.8 in the shake flasks and fermentor, respectively, in which good growth of the fungus was observed during the first 24 h and consumption of proteins dissolved from the rice straw caused the pH to rise later to values between 6.4 and 6.7 (optimal for xylanase production). The xylanases from T. reesei were as effective as Multifect XL, a commercial enzyme preparation, in hydrolyzing ammonia-treated elephant grass.

Index Entries

Xylanase Trichoderma reesei rice straw 

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Alejandro Colina
    • 1
  • Betzabé Sulbarán-de-Ferrer
    • 1
  • Cateryna Aiello
    • 2
  • Alexis Ferrer
    • 1
  1. 1.Laboratorio de Alimentos, Departamento de QuimicaFacultad de CienciasVenezuela
  2. 2.Facultad de IngenieriaUniversidad del ZuliaMaracaiboVenezuela

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