Skip to main content

Saccharification of Marine Microalgae Using Marine Bacteria for Ethanol Production

  • Chapter

Part of the book series: Applied Biochemistry and Biotechnology ((ABAB))

Abstract

The saccharification of marine microalgae using amylase from marine bacteria in saline conditions was investigated. An amylase-producing bacterium, Pseudoalterimonas undina NKMB 0074 was isolated and identified. The green microalga NKG 120701 was determined to have the highest concentration of intracellular carbohydrate and was found from our algal culture stocks. P. undina NKMB 0074 was inoculated into suspensions containing NKG 120701 cells and increasingly reduced suspended sugars with incubation time. Terrestrial amylase and glucoamylase were inactive in saline suspension. Therefore, marine amylase is necessary in saline conditions for successful saccharification of marine microalgae.

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Burgess, J. G., Iwamoto, K., Miura, Y., TaKano, H., and Matsunaga, T. (1993), Appl. Microbiol. Biotechnol. 39, 456–459.

    Article  CAS  Google Scholar 

  2. Miura, Y., Sode, K., Nakamura, N., Matsunaga, N., and Matsunaga, T. (1993), FEMS Microbiol. Lett. 107, 163–168.

    Article  PubMed  CAS  Google Scholar 

  3. Wachi, Y., Burgess, J. G., Takahashi, J., Nakamura, N., and Matsunaga, T. (1995), J. Biotechnol. 2, 210–213.

    CAS  Google Scholar 

  4. Wake, H., Akasaka, A., Umetsu, H., Ozeki, Y., Shimomura, K., and Matsunaga, T. (1992), Plant Cell Rep. 11, 62–65.

    Article  Google Scholar 

  5. Miura, Y., Sode, K., Nakamura, N., and Matsunaga, T. (1993), J. Mar. Biotechnol. 1, 134–146.

    Google Scholar 

  6. Kumazawa, S. and Mitsui A. (1981), Int. J. Hydrogen Energy 6, 339–348.

    Article  CAS  Google Scholar 

  7. Nandi, R. and Sengupta S. (1998), Crit. Rev. Microbiol. 24, 61–84.

    Article  PubMed  CAS  Google Scholar 

  8. Sudo, H., Burgess, J. G., Takemasa, H., Nakamura, N., and Matsunaga, T. (1995), Curr. Microbiol. 30, 219–222.

    Article  CAS  Google Scholar 

  9. Mitsui, A. (1975), in Proceedings of the 3rd Ineternational Ocean Development Conference.

    Google Scholar 

  10. Borowitzka, L.J. and Borowitzka M.A. (1989), in Industrial Production: Method and Economics. Cresswell, R. C., Rees, T. A. V., and Shah, N., eds., Elsevier Applied Science, London, UK, pp. 294–316.

    Google Scholar 

  11. Borowitzka, M. A. (1992), J. Appl. Phycol. 4, 267–279.

    Article  Google Scholar 

  12. Patterson, G. M. L. (1996), J. Sci. Ind. Res. 55, 669–684.

    CAS  Google Scholar 

  13. Mielenz, J. R. (2001), Curr. Opin. Microbiol. 4, 324–329.

    Article  PubMed  CAS  Google Scholar 

  14. Razmovski, R. and Pejin D. (1996), Folia Microbiol. 41, 201–207.

    Article  CAS  Google Scholar 

  15. Joachimsthal, E. and Rogers P. (2000), Appl. Biochem. Biotechnol. 84/86, 343–356.

    Article  Google Scholar 

  16. Krishhan, M., Blanco, M., Shattuck, C. K., Nghiem, N. P., and Davison, B. H. (2000), Appl. Biochem. Biotechnol. 84/86, 525–541.

    Google Scholar 

  17. Lawford, H. and Rousseau, J. (2000), Appl. Biochem. Biotechnol. 84/86, 277–293.

    Google Scholar 

  18. Sandhu, D. and Joshi V. (1994), Indian J. Exp. Biol. 32, 873–876.

    PubMed  CAS  Google Scholar 

  19. Montesinos, T. and Navarro, J.-M. (2000), Enzyme Microbiol. Technol. 27, 362–370.

    Article  CAS  Google Scholar 

  20. Abouzied, M. M. and Reddy, A. C. (1986), Appl. Environ. Microbiol. 52, 1055–1059.

    PubMed  CAS  Google Scholar 

  21. Fumihisa, K., Sawada, T., Nakamura, Y., Ohnaga, M., Godliving, M., and Ushiyama, T. (1998), Appl. Biochem. Biotechnol. 69, 177–189.

    Article  Google Scholar 

  22. Rippka, R., Deruelles, J., Watherbury, J. B., Herdman, M., and Stanier, R. Y. (1979), J. Gen. Microbiol. 111, 1–61.

    Article  Google Scholar 

  23. Miller, G. L.(1959), Anal. Chem. 31, 426–428.

    Article  CAS  Google Scholar 

  24. Lowry, O., Rosebrough, N. J., and Randall, R. J. (1951), J. Biol. Chem. 193, 265–275.

    PubMed  CAS  Google Scholar 

  25. Aristos, A. and Merja, P. (2000), Curr. Opin. Biotechnol. 11, 187–198.

    Article  Google Scholar 

  26. Matsunaga, T. and Izumida, H. (1984), Biotechnol. Bioeng. Symp. 14, 407–418.

    CAS  Google Scholar 

  27. Matsumoto, M., Yoshida, E., Takeyama, H., and Matsunaga, T. (2000), Appl. Biochem. Biotech. 84/86, 51–57.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mitsufumi Matsumoto .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer Science+Business Media New York

About this chapter

Cite this chapter

Matsumoto, M., Yokouchi, H., Suzuki, N., Ohata, H., Matsunaga, T. (2003). Saccharification of Marine Microalgae Using Marine Bacteria for Ethanol Production. In: Davison, B.H., Lee, J.W., Finkelstein, M., McMillan, J.D. (eds) Biotechnology for Fuels and Chemicals. Applied Biochemistry and Biotechnology. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-4612-0057-4_19

Download citation

  • DOI: https://doi.org/10.1007/978-1-4612-0057-4_19

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-4612-6592-4

  • Online ISBN: 978-1-4612-0057-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics