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Expanded Adipose Tissue-Derived Stem Cells for Articular Cartilage Injury Treatment: A Safety and Efficacy Evaluation

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Regenerative Medicine

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Abstract

Non-expanded adipose tissue-derived stem cells (ADSCs) are commonly used in preclinical and clinical articular cartilage injury treatment; however, there are usually insufficient non-expanded ADSCs for transplantation. This research aims to evaluate the safety and efficacy of expanded ADSC transplantation in a mouse model. The stromal vascular fraction from abdominal adipose tissue was subcultured for ten passages to enrich for and expand the number of ADSCs. The safety of expanded ADSCs was assessed by evaluating their “stemness” via ADSC-specific marker expression, the expression of two tumorigenesis-related genes (Oct-3/4 and Nanog) using real-time RT-PCR, and in vivo tumor formation in NOD/SCID mice. ADSC efficacy was determined by assessing their chondrocytic differentiative potential in vitro in cartilage-inducing medium as well as in vivo via injection into a NOD/SCID mice joint failure model. The results showed that expanded ADSCs were negative for Oct-3/4 and Nanog and did not induce tumor formation in mice. Furthermore, ADSCs differentiated into chondrocytes, both in vitro and in vivo, and enhanced the regeneration of articular cartilage in NOD/SCID mice as compared with the control. These results confirm that expanded ADSCs are safe and effective for the treatment of injured articular cartilage and offer a promising therapy for degenerative cartilaginous diseases.

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Authors and Affiliations

  1. Laboratory of Stem Cell Research and Application, University of Science¸ Vietnam National University, Ho Chi Minh City, 227 Nguyen Van Cu, District 5, Ho Chi Minh City, 08000, Vietnam

    Phuc Van Pham PhD, Ngoc Bich Vu, Nhung Hai Truong MSc, Dung Minh Le BS & Ngoc Kim Phan PhD

  2. Orthopaedic Department, University Medical Center, Ho Chi Minh City, University of Medicine and Pharmacology, Ho Chi Minh City, 215- 217 Hong Bang, District 5, Ho Chi Minh City, 08000, Vietnam

    Khanh Hong Thien Bui MD, PhD

  3. Department of Pathology, University of Medicine and Pharmacy, Ho Chi Minh City, 217 Hồng Bàng, 11th Ward, District 5, Ho Chi Minh City, 08000, Vietnam

    Dat Quoc Ngo MD, PhD & Thao Thi-Phuong Doan PhD

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  1. Calcutta School of Tropical Medicine, Kolkata, India

    Niranjan Bhattacharya

  2. Boston University School of Medicine, Jamaica Plain, Massachusetts, USA

    Phillip George Stubblefield

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Van Pham, P. et al. (2015). Expanded Adipose Tissue-Derived Stem Cells for Articular Cartilage Injury Treatment: A Safety and Efficacy Evaluation. In: Bhattacharya, N., Stubblefield, P. (eds) Regenerative Medicine. Springer, London. https://doi.org/10.1007/978-1-4471-6542-2_11

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