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New Frontiers in the Production of Biodiesel: Biodiesel Derived from Macro and Microorganisms

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Liquid Biofuels: Emergence, Development and Prospects

Part of the book series: Lecture Notes in Energy ((LNEN,volume 27))

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Abstract

The biodiesel industry is gaining interest in the past years due to the depletion of the easily extracted petroleum, the increasing demand to the automotive market, and the environmental damage. It is acknowledged that the main obstacle to biodiesel marketing is the cost of production, which is mostly due to the price of the raw material (usually vegetable oils). In this way, the goal is to provide low-cost raw materials. This may be achieved by feedstocks that do not require arable land, do not depend on growing seasons, and that give added value to waste, helping also to its recycling. In this way, oleaginous organisms may be considered an alternative feedstock for the biodiesel industry, as they meet all the previous requirements. This chapter presents the state of the art and the main characteristics of the oil and biodiesel provided by macroorganism (insects) and microorganism (bacteria, filamentous fungi, and yeasts).

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References

  • Ageitos JM, Vallejo JA, Veiga-Crespo P, Villa TG (2011) Oily yeasts as oleaginous cell factories. Appl Microbiol Biotechnol 90(4):1219–1227

    Article  Google Scholar 

  • Alvarez HM, Steinbuchel A (2002) Triacylglycerols in prokaryotic microorganisms. Appl Microbiol Biotechnol 60(4):367–376

    Article  Google Scholar 

  • Azocar L, Ciudad G, Heipieper HJ, Navia R (2010) Biotechnological processes for biodiesel production using alternative oils. Appl Microbiol Biotechnol 88(3):621–636

    Article  Google Scholar 

  • Bellou S, Moustogianni A, Makri A, Aggelis G (2012) Lipids Containing Polyunsaturated Fatty Acids Synthesized by Zygomycetes Grown on Glycerol. Appl Biochem Biotechnol 166(1):146–158

    Article  Google Scholar 

  • Belluco S, Losasso C, Maggioletti M, Alonzi CC, Paoletti MG, Ricci A (2013) Edible insects in a food safety and nutritional perspective: a critical review. Compr Rev Food Sci Food Safety 12(3):296–313

    Article  Google Scholar 

  • Beopoulos A, Nicaud JM, Gaillardin C (2011) An overview of lipid metabolism in yeasts and its impact on biotechnological processes. Appl Microbiol Biotechnol 90(4):1193–1206

    Article  Google Scholar 

  • Chatzifragkou A, Makri A, Belka A, Bellou S, Mavrou M, Mastoridou M, Mystrioti P, Onjaro G, Aggelis G, Papanikolaou S (2011) Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species. Energy 36(2):1097–1108

    Article  Google Scholar 

  • Deandres C, Espuny MJ, Robert M, Mercade ME, Manresa A, Guinea J (1991) Cellular lipid-accumulation by Pseudomonas aeruginosa 44T1. Appl Microbiol Biotechnol 35(6):813–816

    Article  Google Scholar 

  • Demirbas A (2009) Progress and recent trends in biodiesel fuels. Energ Conv Manag 50(1):14–34

    Article  Google Scholar 

  • Dey P, Maiti MK (2013) Molecular characterization of a novel isolate of Candida tropicalis for enhanced lipid production. J Appl Microbiol 114(5):1357–1368

    Article  Google Scholar 

  • Dorado MP, Cruz F, Palomar JM, Lopez FJ (2006) An approach to the economics of two vegetable oil-based biofuels in Spain. Renewable Energ 31(8):1231–1237

    Article  Google Scholar 

  • Eberly JO, Ringelberg DB, Indest KJ (2013) Physiological characterization of lipid accumulation and in vivo ester formation in Gordonia sp KTR9. J Ind Microbiol Biotechnol 40(2):201–208

    Article  Google Scholar 

  • Economou CN, Aggelis G, Pavlou S, Vayenas DV (2011) Single cell oil production from rice hulls hydrolysate. Bioresour Technol 102(20):9737–9742

    Article  Google Scholar 

  • Galafassi S, Cucchetti D, Pizza F, Franzosi G, Bianchi D, Compagno C (2012) Lipid production for second generation biodiesel by the oleaginous yeast Rhodotorula graminis. Bioresour Technol 111:398–403

    Article  Google Scholar 

  • Gao DF, Zeng JJ, Zheng YB, Yu XC, Chen SL (2013) Microbial lipid production from xylose by Mortierella isabellina. Bioresour Technol 133:315–321

    Article  Google Scholar 

  • Gong ZW, Wang Q, Shen HW, Hu CM, Jin GJ, Zhao ZBK (2012) Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production. Bioresour Technol 117:20–24

    Article  Google Scholar 

  • Gouda MK, Omar SH, Aouad LM (2008) Single cell oil production by Gordonia sp DG using agro-industrial wastes. World J Microbiol Biotechnol 24(9):1703–1711

    Article  Google Scholar 

  • Gui MM, Lee KT, Bhatia S (2008) Feasibility of edible oil vs. non-edible oil vs. waste edible oil as biodiesel feedstock. Energy 33(11):1646–1653

    Article  Google Scholar 

  • Janda K, Kristoufek L, Zilberman D (2012) Biofuels: policies and impacts. Agric Econ 58(8):372–386

    Google Scholar 

  • Kalscheuer R, Stolting T, Steinbuchel A (2006) Microdiesel: Escherichia coli engineered for fuel production. Microbiology-(UK) 152:2529–2536

    Google Scholar 

  • Karimi K, Zamani A (2013) Mucor indicus: biology and industrial application perspectives: a review. Biotechnol Adv 31(4):466–481

    Article  Google Scholar 

  • Khot M, Kamat S, Zinjarde S, Pant A, Chopade B, RaviKumar A (2012) Single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel. Microb Cell Fact 11

    Google Scholar 

  • Kosa M, Ragauskas AJ (2011) Lipids from heterotrophic microbes: advances in metabolism research. Trends Biotechnol 29(2):53–61

    Article  Google Scholar 

  • Kosa M, Ragauskas AJ (2012) Bioconversion of lignin model compounds with oleaginous Rhodococci. Appl Microbiol Biotechnol 93(2):891–900

    Article  Google Scholar 

  • Kumar AK, Vatsyayan P, Goswami P (2010) Production of lipid and fatty acids during growth of Aspergillus terreus on hydrocarbon substrates. Appl Biochem Biotechnol 160(5):1293–1300

    Article  Google Scholar 

  • Leung D, Yang DP, Li ZX, Zhao ZM, Chen JP, Zhu LP (2012) Biodiesel from Zophobas morio larva oil: process optimization and FAME characterization. Ind Eng Chem Res 51(2):1036–1040

    Article  Google Scholar 

  • Li Q, Du W, Liu D (2008) Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol 80(5):749–756

    Article  Google Scholar 

  • Li Q, Zheng L, Cai H, Garza E, Yu Z, Zhou S (2011a) From organic waste to biodiesel: Black soldier fly, Hermetia illucens, makes it feasible. Fuel 90(4):1545–1548

    Article  Google Scholar 

  • Li Q, Zheng L, Qiu N, Cai H, Tomberlin JK, Yu Z (2011b) Bioconversion of dairy manure by black soldier fly (diptera: Stratiomyidae) for biodiesel and sugar production. Waste Manag 31(6):1316–1320

    Article  Google Scholar 

  • Li Z, Yang D, Huang M, Hu X, Shen J, Zhao Z, Chen J (2012) Chrysomya megacephala (Fabricius) larvae: a new biodiesel resource. Appl Energ 94:349–354

    Article  Google Scholar 

  • Liang YN, Cui Y, Trushenski J, Blackburn JW (2010) Converting crude glycerol derived from yellow grease to lipids through yeast fermentation. Bioresour Technol 101(19):7581–7586

    Article  Google Scholar 

  • Liu B, Zhao Z (2007) Biodiesel production by direct methanolysis of oleaginous microbial biomass. J Chem Technol Biotechnol 82(8):775–780

    Article  Google Scholar 

  • Lu X, Vora H, Khosla C (2008) Overproduction of free fatty acids in E. coli: implications for biodiesel production. Metab Eng 10(6):333–339

    Article  Google Scholar 

  • Lunin VV, Sergeeva YE, Galanina LA, Mysyakina IS, Ivashechkin AA, Bogdan VI, Feofilova EP (2013) Biodiesel fuel production from lipids of filamentous fungi. Appl Biochem Microbiol 49(1):46–52

    Article  Google Scholar 

  • Manzano-Agugliaro F, Sanchez-Muros MJ, Barroso FG, Martínez-Sánchez A, Rojo S, Pérez-Banon C (2012) Insects for biodiesel production. Renew Sustain Energy Rev 16(6):3744–3753

    Article  Google Scholar 

  • Mitra D, Rasmussen ML, Chand P, Chintareddy VR, Yao L, Grewell D, Verkade JG, Wang T, van Leeuwen J (2012) Value-added oil and animal feed production from corn-ethanol stillage using the oleaginous fungus Mucor circinelloides. Bioresour Technol 107:368–375

    Article  Google Scholar 

  • Muniraj IK, Xiao L, Hu Z, Zhan X, Shi J (2013) Microbial lipid production from potato processing wastewater using oleaginous filamentous fungi Aspergillus oryzae. Water Res 47(10):3477–3483

    Article  Google Scholar 

  • Neste oil (2012) The only way is forward, annual report 2012

    Google Scholar 

  • Patnayak S, Sree A (2005) Screening of bacterial associates of marine sponges for single cell oil and PUFA. Lett Appl Microbiol 40(5):358–363

    Article  Google Scholar 

  • Pinzi S, Gandía LM, Arzamendi G, Ruiz JJ, Dorado MP (2011) Influence of vegetable oils fatty acid composition on reaction temperature and glycerides conversion to biodiesel during transesterification. Bioresour Technol 102(2):1044–1050

    Article  Google Scholar 

  • Pinzi S, Garcia IL, Lopez-Gimenez FJ, de Castro MDL, Dorado G, Dorado MP (2009) The ideal vegetable oil-based biodiesel composition: a review of social, economical and technical implications. Energ Fuels 23:2325–2341

    Article  Google Scholar 

  • Raksakantong P, Meeso N, Kubola J, Siriamornpun S (2010) Fatty acids and proximate composition of eight Thai edible terricolous insects. Food Res Int 43(1):350–355

    Article  Google Scholar 

  • Ramos-Elorduy J (2008) Energy supplied by edible insects from Mexico and their nutritional and ecological importance. Ecol Food Nutr 47(3):280–297

    Article  Google Scholar 

  • Ruan Z, Zanotti M, Wang X, Ducey C, Liu Y (2012) Evaluation of lipid accumulation from lignocellulosic sugars by Mortierella isabellina for biodiesel production. Bioresour Technol 110:198–205

    Article  Google Scholar 

  • Rucker J, Paul J, Pfeifer B, Lee K (2013) Engineering E. coli for triglyceride accumulation through native and heterologous metabolic reactions. Appl Microbiol Biotechnol 97(6):2753–2759

    Article  Google Scholar 

  • Ruenwai R, Cheevadhanarak S, Laoteng K (2009) Overexpression of acetyl-CoA carboxylase gene of Mucor rouxii enhanced fatty acid content in Hansenula polymorpha. Mol Biotechnol 42(3):327–332

    Article  Google Scholar 

  • Rumpold BA, Schluter OK (2013) Nutritional composition and safety aspects of edible insects. Mol Nutr Food Res 57(5):802–823

    Article  Google Scholar 

  • Ryu B-G, Kim J, Kim K, Choi Y-E, Han J-I, Yang J-W (2013) High-cell-density cultivation of oleaginous yeast Cryptococcus curvatus for biodiesel production using organic waste from the brewery industry. Bioresour Technol 135:357–364

    Article  Google Scholar 

  • Saenge C, Cheirsilp B, Suksaroge TT, Bourtoom T (2011) Potential use of oleaginous red yeast Rhodotorula glutinis for the bioconversion of crude glycerol from biodiesel plant to lipids and carotenoids. Process Biochem 46(1):210–218

    Article  Google Scholar 

  • Sankh S, Thiru M, Saran S, Rangaswamy V (2013) Biodiesel production from a newly isolated Pichia kudriavzevii strain. Fuel 106:690–696

    Article  Google Scholar 

  • Shi SB, Valle-Rodriguez JO, Siewers V, Nielsen J (2011) Prospects for microbial biodiesel production. Biotechnol J 6(3):277–285

    Article  Google Scholar 

  • St-Hilaire S, Cranfill K, McGuire MA, Mosley EE, Tomberlin JK, Newton L, Sealey W, Sheppard C, Irving S (2007) Fish offal recycling by the black soldier fly produces a foodstuff high in omega-3 fatty acids. J World Aquaculture Soc 38(2):309–313

    Article  Google Scholar 

  • Taha EM, Omar O, Yusoff WMW, Hamid AA (2010) Lipid biosynthesis in Cunninghamella bainieri 2A1 in N-limited and N-excess media. Ann Microbiol 60(4):615–622

    Article  Google Scholar 

  • Uckun Kiran E, Salakkam A, Trzcinski AP, Bakir U, Webb C (2012) Enhancing the value of nitrogen from rapeseed meal for microbial oil production. Enzyme Microbial Technol 50(6–7):337–342

    Article  Google Scholar 

  • Uckun Kiran E, Trzcinski A, Webb C (2013) Microbial oil produced from biodiesel by-products could enhance overall production. Bioresour Technol 129:650–654

    Article  Google Scholar 

  • Venkata Subhash G, Venkata Mohan S (2011) Biodiesel production from isolated oleaginous fungi Aspergillus sp. using corncob waste liquor as a substrate. Bioresour Technol 102 (19):9286–9290

    Google Scholar 

  • Vicente G, Bautista LF, Rodríguez R, Gutiérrez FJ, Sádaba I, Ruiz-Vázquez RM, Torres-Martínez S, Garre V (2009) Biodiesel production from biomass of an oleaginous fungus. Biochem Eng J 48(1):22–27

    Article  Google Scholar 

  • Vongsangnak W, Ruenwai R, Tang X, Hu X, Zhang H, Shen B, Song Y, Laoteng K (2013) Genome-scale analysis of the metabolic networks of oleaginous Zygomycete fungi. Gene 521(1):180–190

    Article  Google Scholar 

  • Wahlen BD, Morgan MR, McCurdy AT, Willis RM, Morgan MD, Dye DJ, Bugbee B, Wood BD, Seefeldt LC (2012) Biodiesel from microalgae, yeast, and bacteria: engine performance and exhaust emissions. Energ Fuels 27(1):220–228

    Article  Google Scholar 

  • Wu SG, Hu CM, Jin GJ, Zhao X, Zhao ZK (2010) Phosphate-limitation mediated lipid production by Rhodosporidium toruloides. Bioresour Technol 101(15):6124–6129

    Article  Google Scholar 

  • Wynn JP, Hamid ABA, Ratledge C (1999) The role of malic enzyme in the regulation of lipid accumulation in filamentous fungi. Microbiology-Uk 145:1911–1917

    Article  Google Scholar 

  • Xia CJ, Zhang JG, Zhang WD, Hu B (2011) A new cultivation method for microbial oil production: cell pelletization and lipid accumulation by Mucor circinelloides. Biotechnol Biofuels 4

    Google Scholar 

  • Xu JY, Du W, Zhao XB, Zhang GL, Liu DH (2013) Microbial oil production from various carbon sources and its use for biodiesel preparation. Biofuels Bioprod Biorefining 7(1):65–77

    Article  Google Scholar 

  • Xu JY, Zhao XB, Wang WC, Du W, Liu DH (2012) Microbial conversion of biodiesel byproduct glycerol to triacylglycerols by oleaginous yeast Rhodosporidium toruloides and the individual effect of some impurities on lipid production. Biochem Eng J 65:30–36

    Article  Google Scholar 

  • Yu XC, Zheng YB, Dorgan KM, Chen SL (2011) Oil production by oleaginous yeasts using the hydrolysate from pretreatment of wheat straw with dilute sulfuric acid. Bioresour Technol 102(10):6134–6140

    Article  Google Scholar 

  • Zeng J, Zheng Y, Yu X, Yu L, Gao D, Chen S (2013) Lignocellulosic biomass as a carbohydrate source for lipid production by Mortierella isabellina. Bioresour Technol 128:385–391

    Article  Google Scholar 

  • Zhao X, Hu CM, Wu SG, Shen HW, Zhao ZK (2011) Lipid production by Rhodosporidium toruloides Y4 using different substrate feeding strategies. J Ind Microbiol Biotechnol 38(5):627–632

    Article  Google Scholar 

  • Zhao XB, Peng F, Du W, Liu CM, Liu DH (2012) Effects of some inhibitors on the growth and lipid accumulation of oleaginous yeast Rhodosporidium toruloides and preparation of biodiesel by enzymatic transesterification of the lipid. Bioprocess Biosyst Eng 35(6):993–1004

    Article  Google Scholar 

  • Zheng L, Hou Y, Li W, Yang S, Li Q, Yu Z (2012a) Biodiesel production from rice straw and restaurant waste employing black soldier fly assisted by microbes. Energy 47(1):225–229

    Article  Google Scholar 

  • Zheng L, Hou Y, Li W, Yang S, Li Q, Yu Z (2013) Exploring the potential of grease from yellow mealworm beetle (Tenebrio molitor) as a novel biodiesel feedstock. Appl Energy 101:618–621

    Article  Google Scholar 

  • Zheng YB, Yu XC, Zeng JJ, Chen SL (2012b) Feasibility of filamentous fungi for biofuel production using hydrolysate from dilute sulfuric acid pretreatment of wheat straw. Biotechnol Biofuels 5

    Google Scholar 

  • Zikou E, Chatzifragkou A, Koutinas AA, Papanikolaou S (2013) Evaluating glucose and xylose as cosubstrates for lipid accumulation and γ-linolenic acid biosynthesis of Thamnidium elegans. J Appl Microbiol 114(4):1020–1032

    Article  Google Scholar 

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Acknowledgments

This research was supported by the Spanish Ministry of Education and Science (ENE2010-15159) and the Andalusian Economy, Innovation and Enterprise Council, Spain (TEP-4994).

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Leiva-Candia, D.E., Dorado, M.P. (2014). New Frontiers in the Production of Biodiesel: Biodiesel Derived from Macro and Microorganisms. In: Domingos Padula, A., Silveira dos Santos, M., Benedetti Santos, O., Borenstein, D. (eds) Liquid Biofuels: Emergence, Development and Prospects. Lecture Notes in Energy, vol 27. Springer, London. https://doi.org/10.1007/978-1-4471-6482-1_11

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