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Molecular Biology of Valvular Heart Disease pp 27–33Cite as

Experimental Model of Aortic Valve Calcification to Induce Osteoblast Differentiation

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Abstract

Over the past 25 years, the development of an in vivo model to test for calcific aortic valve disease has been challenging. The understanding of the biology, imaging, and duration of exposure to risk factors, have been the cornerstone for defining the different stages of osteoblast differentiation. The first experimental was high cholesterol-diet rabbit model. The studies published demonstrated apoptosis [1], cell proliferation [2], and atherosclerosis [1, 3–6] along the aortic valve surface. These models all include short time diet experiments to define the early atherosclerotic findings in the valve. The next level of experimentation includes testing the diet for 6 months. This duration of diet provides the time necessary for the valve to mineralize and to calcify.

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Authors and Affiliations

  1. Division of Biochemistry and Molecular Biology, Mayo Clinic, 200 First St SW, Rochester, MN, 55905, USA

    Nalini M. Rajamannan MD, Muzaffer Cicek, John R. Hawse, Thomas C. Spelsberg Phd & Malayannan Subramaniam

Authors
  1. Nalini M. Rajamannan MD
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  2. Muzaffer Cicek
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  3. John R. Hawse
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  4. Thomas C. Spelsberg Phd
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  5. Malayannan Subramaniam
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Corresponding author

Correspondence to Nalini M. Rajamannan MD .

Editor information

Editors and Affiliations

  1. Biochemistry & Molecular Biology Dept., Mayo Clinic, Rochester, Minnesota, USA

    Nalini M. Rajamannan

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© 2014 Springer-Verlag London

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Rajamannan, N.M., Cicek, M., Hawse, J.R., Spelsberg, T.C., Subramaniam, M. (2014). Experimental Model of Aortic Valve Calcification to Induce Osteoblast Differentiation. In: Rajamannan, N. (eds) Molecular Biology of Valvular Heart Disease. Springer, London. https://doi.org/10.1007/978-1-4471-6350-3_4

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  • DOI: https://doi.org/10.1007/978-1-4471-6350-3_4

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  • Publisher Name: Springer, London

  • Print ISBN: 978-1-4471-6349-7

  • Online ISBN: 978-1-4471-6350-3

  • eBook Packages: MedicineMedicine (R0)

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