Abstract
Monitoring of allograft rejection is critical to be able to provide proper immunosuppressive treatment following allotransplantation. Skin biopsy is needed to prove and to grade of rejection according to the current protocols, but it is an invasive and time-consuming method. An experimental study was performed to describe a non-invasive monitoring of allograft rejection. Five skin-containing vascularized composite allotransplantations were performed on rats, and allograft rejection was evaluated with reflectance confocal microscopy and also with skin biopsy. All data obtained from the study were evaluated and compared statistically. Reflectance confocal microscopy is presented as a new non-invasive rejection monitoring technique, and the new allograft rejection scoring system is recommended based on the results of this experimental study in this chapter.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Murray JE. Organ transplantation (skin, kidney, heart) and the plastic surgeon. Plast Reconstr Surg. 1971;47(5):425–31.
Lee WP, Yaremchuk MJ, Pan YC, Randolph MA, Tan CM, Weiland AJ. Relative antigenicity of components of a vascularized limb allograft. Plast Reconstr Surg. 1991;87(3):401–11.
Cendales LC, Breidenbach 3rd WC. Hand transplantation. Hand Clin. 2001;17(3):499–510, x.
Cendales LC, Kanitakis J, Schneeberger S, Burns C, Ruiz P, Landin L, et al. The Banff 2007 working classification of skin-containing composite tissue allograft pathology. Am J Transplant. 2008;8(7):1396–400.
Petruzzo P, Lanzetta M, Dubernard JM, Landin L, Cavadas P, Margreiter R, et al. The international registry on hand and composite tissue transplantation. Transplantation. 2010;90(12):1590–4.
Cendales LC, Kirk AD, Moresi JM, Ruiz P, Kleiner DE. Composite tissue allotransplantation: classification of clinical acute skin rejection. Transplantation. 2005;80(12):1676–80.
Ravindra KV, Buell JF, Kaufman CL, Blair B, Marvin M, Nagubandi R, et al. Hand transplantation in the United States: experience with 3 patients. Surgery. 2008;144(4):638–43; discussion 43–4.
Hofmann-Wellenhof R, Wurm EM, Ahlgrimm-Siess V, Richtig E, Koller S, Smolle J, et al. Reflectance confocal microscopy – state-of-art and research overview. Semin Cutan Med Surg. 2009;28(3):172–9.
Petruzzo P, Kanitakis J, Badet L, Pialat JB, Boutroy S, Charpulat R, et al. Long-term follow-up in composite tissue allotransplantation: in-depth study of five (hand and face) recipients. Am J Transplant. 2011;11(4):808–16.
Kanitakis J. The challenge of dermatopathological diagnosis of composite tissue allograft rejection: a review. J Cutan Pathol. 2008;35(8):738–44.
Astner S, Gonzalez E, Cheung A, Rius-Diaz F, Gonzalez S. Pilot study on the sensitivity and specificity of in vivo reflectance confocal microscopy in the diagnosis of allergic contact dermatitis. J Am Acad Dermatol. 2005;53(6):986–92.
Basaran YK, Gurel MS, Erdemir AT, Turan E, Yurt N, Bagci IS. Evaluation of the response to treatment of psoriasis vulgaris with reflectance confocal microscopy. Skin Res Technol. 2014. doi: 10.1111/srt.12150. [Epub ahead of print].
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag London
About this chapter
Cite this chapter
Karagoz, H., Gurel, M.S., Zor, F. (2015). Non-invasive Monitoring of Skin-Containing Vascularized Composite Allotransplantation. In: Siemionow, M. (eds) Plastic and Reconstructive Surgery. Springer, London. https://doi.org/10.1007/978-1-4471-6335-0_42
Download citation
DOI: https://doi.org/10.1007/978-1-4471-6335-0_42
Published:
Publisher Name: Springer, London
Print ISBN: 978-1-4471-6334-3
Online ISBN: 978-1-4471-6335-0
eBook Packages: MedicineMedicine (R0)