A Xenotransplantation Model for Vascularized Composite Transplantation



Increased demand for solid transplant organs yielded development of xenotransplantation. Thus, the main research area of xenotransplantation is the transplantation of vital organs such as heart, kidney and liver. However, xenotransplantation of vascularized composite tissues have not been attempted yet. In this chapter, a concordant vascularized composite xenotransplantation model is described. This composite tissue xenotransplantation model was successfully used for preservation of a composite tissue block for a certain time period, which called composite tissue xenopreservation. However, further studies of the xenotransplantation of composite tissues are needed.


Xenotransplantation Tissue preservation Composite tissue transplantation Composite tissue xenotransplantation Groin flap 


  1. 1.
    Saint-Cyr M, Wong C, Buchel EW, Colohan S, Pederson W. Free tissue transfers and replantation. Plast Reconstr Surg. 2012;130:858e–78.PubMedCrossRefGoogle Scholar
  2. 2.
    Mazur P. Freezing of living cells: mechanisms and implications. Am J Physiol. 1984;247:125–42.Google Scholar
  3. 3.
    Bakhach J. The cryopreservation of composite tissues: principles and recent advancement on cryopreservation of different type of tissues. Organogenesis. 2009;5:119–26.PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Arakawa T, Carpenter JF, Kita YA, Crowe JH. The basis for toxicity of certain cryoprotectants: a hypothesis. Cryobiology. 1990;27:401–15.CrossRefGoogle Scholar
  5. 5.
    Cui X, Gao D, Fink B, Vasconez HC, Rinker B. Cryopreservation of composite tissues and transplantation: preliminary studies. Cryobiology. 2007;55:295–304.PubMedCrossRefGoogle Scholar
  6. 6.
    Rinker B, Cui XD, Fink BF, Gao DY, Vasconez HC. Cryopreservation of composite tissue flaps: experimental studies in the rat. Ann Plast Surg. 2007;58:656–60.PubMedCrossRefGoogle Scholar
  7. 7.
    Zhang F, Attkis KJ, Walker M, Buncke HJ. Effect of cryopreservation on survival of composite tissue grafts. J Reconstr Microsurg. 1998;14:559–64.PubMedCrossRefGoogle Scholar
  8. 8.
    Cooper DK. A brief history of cross-species organ transplantation. Proc (Bayl Univ Med Cent). 2012;25:49–57.Google Scholar
  9. 9.
    Galili U, Wang L, LaTemple DC, Radic MZ. The natural anti-gal antibody. Subcell Biochem. 1999;32:79–106.PubMedGoogle Scholar
  10. 10.
    Taniguchi S, Cooper DK. Clinical xenotransplantation: past, present and future. Ann R Coll Surg Engl. 1997;79:13–9.PubMedCentralPubMedGoogle Scholar
  11. 11.
    Miyagawa S, Hirose H, Shirakura R, Naka Y, Nakata S, Kawashima Y, Seya T, Matsumoto M, Uenaka A, Kitamura H. The mechanism of discordant xenograft rejection. Transplantation. 1988;46:825–30.PubMedCrossRefGoogle Scholar
  12. 12.
    Fryer JP, Leventhal JR, Dalmasso AP, Chen S, Simone PA, Jessurun J, Sun LH, Reinsmoen NL, Matas AJ. Cellular rejection in discordant xenografts when hyperacute rejection is prevented: analysis using adoptive and passive transfer. Transpl Immunol. 1994;2:87–93.PubMedCrossRefGoogle Scholar
  13. 13.
    Petry JJ, Wortham KA. The anatomy of the epigastric flap in the experimental rat. Plast Reconstr Surg. 1984;74:410–3.PubMedCrossRefGoogle Scholar
  14. 14.
    Rinker B, Cui XD, Cibull ML, Fink BF, Gao DY, Vasconez HC. Cryopreservation of composite tissue transplants. Hand (N Y). 2008;3:17–23.CrossRefGoogle Scholar
  15. 15.
    Sachs DH, Sykes M, Robson SC, Cooper DK. Xenotransplantation. Adv Immunol. 2001;79:129–223.PubMedCrossRefGoogle Scholar
  16. 16.
    Bersztel A, Lorant T, Björkland A, Johnsson C, Tufveson G. Antibody responses to xenogenic antigens – a study in the mouse-to-rat system. Tissue Antigens. 2006;68:483–8.PubMedCrossRefGoogle Scholar

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© Springer-Verlag London 2015

Authors and Affiliations

  1. 1.Department of Plastic and Reconstructive SurgeryGulhane Military Medical AcademyAnkaraTurkey
  2. 2.Department of Plastic and Reconstructive SurgeryGulhane Military Medical Academy, Haydarpasa Training HospitalIstanbulTurkey

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