Vascular Thymus and Hind Limb Allotransplantation Model

  • Turgut Ortak


The immunology of composite tissue allotransplantation is complex because the grafts are composed of a variety of tissues, such as skin, fat, muscle, nerves, lymph nodes, bone, cartilage, ligaments, and bone marrow, representing different antigenicities. Composite tissue allotransplantation transplants are not routinely performed today because of the need for lifelong nonspecific immunosuppressive agents to prevent the immune system from rejecting the foreign tissue. Researchers have been actively looking for alternative methods that can induce lifelong tolerance using less toxic regimens. One such method could be tolerance, chimeric rat model by transplanting both thymus and hind limb of the one specie rat as donor to the other specie rat as recipient.


Composite tissue allotransplantation Vascular Thymus Transplantation Chimerism Chimeric rat model Allotransplantation 


  1. 1.
    Gordon CR, Nazzal J, Lozano-Calderan SA, Lee SG, Lee WP, Siemionow M, et al. From experimental rat hindlimb to clinical face composite tissue allotransplantation: historical background and current status. Microsurgery. 2006;26:566–72. doi: 10.1002/micr.20296.PubMedCrossRefGoogle Scholar
  2. 2.
    Gorantla V, Maldonado C, Frank J, Barker JH. Composite tissue allotransplantation (CTA): current status and future insights. Eur J Trauma. 2001;27:267–74.Google Scholar
  3. 3.
    Siemionow M, Ozer K. Advances in composite tissue allograft transplantation as related to the hand and upper extremity. J Hand Surg Am. 2002;27:565–80. doi: 10.1053/jhsu.2002.34367.PubMedCrossRefGoogle Scholar
  4. 4.
    Siemionow M, Papay F, Kulahci Y, Djohan R, Hammert W, Hendrickson M, et al. Coronal-posterior approach for face/scalp flap harvesting in preparation for face transplantation. J Reconstr Microsurg. 2006;22:399–405. doi: 10.1055/s-2006-947693.PubMedCrossRefGoogle Scholar
  5. 5.
    Siemionow M, Bozkurt M, Kulahci Y. Current status of composite tissue allotransplantation. Handchir Mikrochir Plast Chir. 2007;39:145–55.PubMedCrossRefGoogle Scholar
  6. 6.
    Lee WP, Yaremchuk MJ, Pan YC, Randolph MA, Tan CM, Weiland AJ. Relative antigenicity of components of a vascularized limb allograft. Plast Reconstr Surg. 1991;87:401–11.PubMedCrossRefGoogle Scholar
  7. 7.
    Foster RD, Fan L, Neipp M, Kaufman C, McCalmont T, Ascher N, et al. Donor specific tolerance induction in composite tissue allografts. Am J Surg. 1998;176(5):418–21.PubMedCrossRefGoogle Scholar
  8. 8.
    Gammie JS, Li S, Colson YL, Demetris AJ, Neipp M, Ildstad ST, Pham SM. A partial conditioning strategy for achieving mixed chimerism in rat: tacrolimus and anti-lymphocyte serum substantially reduce the minimum radiation dose for engraftment. Exp Hematol. 1998;26(10):927–35.PubMedGoogle Scholar
  9. 9.
    Gyopar AF, Loertscher R, Guttman RD, Tchervenkov JI. A comparison among the effects of donor-specific whole blood, bone marrow, and spleen leukocytes on allograft survival when combined with cyclosporine. Transplantation. 1993;55(5):1199–202.PubMedCrossRefGoogle Scholar
  10. 10.
    Hewitt CW, Llull R, Patel MP, et al. Mechanisms of unresponsiveness associated with pretransplant blood transfusion-cyclosporine-induced mixed lymphocyte chimerism. Transpl Int. 1994;7:559.CrossRefGoogle Scholar
  11. 11.
    Adanali G, Ozer K, Siemionow M. Early and late effects of ischemic preconditioning on microcirculation of skeletal muscle flaps. Plast Reconstr Surg. 2002;109(4):1344–51.PubMedCrossRefGoogle Scholar
  12. 12.
    Küntscher MV, Kastell T, Sauerbier M, Nobiling R, Gebhard MM, Germann G. Acute remote ischemic preconditioning on a rat cremasteric muscle flap model. Microsurgery. 2002;22(6):221–6. doi: 10.1002/micr.10041.PubMedCrossRefGoogle Scholar
  13. 13.
    Siemionow M, Klimczak A. Chimerism-based experimental models for tolerance induction in vascularized composite allografts: Cleveland Clinic Research Experience Clinical and Developmental Immunology 2013; Article ID 831410, 12 pages.Google Scholar
  14. 14.
    Siemionow M, Izycki D, Ozer K, Ozmen S, Klimczak A. Role of thymus in operational tolerance induction in limb allograft transplant model. Transplantation. 2006;81(11):1568–76. doi: 10.1097/ Scholar

Copyright information

© Springer-Verlag London 2015

Authors and Affiliations

  1. 1.Department of Plastic and Reconstructive SurgerySüleyman Demirel University Medical Faculty, Research and Training HospitalIspartaTurkey

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