Abstract
The use of intravital microscopy in experimental transplantation research has been popular for decades. Siemionow et al. have reported several in vivo studies on the microcirculation of allograft rejection and ischemia/ reperfusion injury using a rat cremaster muscle model. In these studies, the cremaster muscle was used not only as a free flap, but also incorporated into hind-limb allografts as part of the composite tissue. As a result, a pattern of leukocyte trafficking, and sequence of hemodynamic changes were established during the acute rejection of composite tissue allografts. Rat model however, presents certain obstacles. Genetically engineered transgenic and knock-out rat strains are not readily available. Also, number of monoclonal antibodies and reagents are limited in rats. To overcome those limitations, we developed mouse cremaster muscle allograft model. In this chapter, you will find relevant technical details, advantages, and challenges of the model.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Huang CD, Chow SP, Chan CW. Experience with anastomoses of arteries of approximately 0.20 mm in diameter. Plast Reconstr Surg. 1982;69:299–305.
Ozer K, Zielinski M, Unsal M, Siemionow M. Development of mouse cremaster transplantation model for intravital microscopic evaluation. Microcirculation. 2002;9(6):487–96.
Siemionow M, Andreasen T, Lister G. Microcirculatory response to surgical trauma in composite tissue transfer. J Reconstr Microsurg. 1995;11:7–13.
Siemionow M, Kimori L, Lister G. Microcirculatory hemodynamics during acute phase of free vascularized muscle allograft rejection. Ann Plast Surg. 1998;41:275–82.
Ozer K, Adanali G, Zins J, Siemionow M. In vivo microscopic assessment of cremasteric microcirculation during hindlimb allograft rejection in rats. Plast Reconstr Surg. 1999;103:1949–56.
Chandrasekharan UM, Siemionow M, Unsal M, Yang L, Poptic E, Bohn J, Ozer K, Zhou Z, Howe PH, Penn M, DiCorleto PE. Tumor necrosis factor alpha (TNF-alpha) receptor-II is required for TNF-alpha-induced leukocyte-endothelial interaction in vivo. Blood. 2007;109(5):1938–44.
Schultz LD. Immunological mutants of the mouse. Am J Anat. 1991;191:303–11.
Endrich B, Hammersen F, Goetz A, Messmer K. Microcirculatory blood flow, capillary morphology and local oxygen pressure of the hamster amelanotic melanoma A-Mel-3. J Natl Cancer Inst. 1982;68:475–85.
Post S, Rentsch M, Gonzalez AP, et al. Effects of Carolina rinse and adenosine rinse on microvascular perfusion and intrahepatic leukocyte-endothelium interaction after liver transplantation in the rat. Transplantation. 1993;55:972–7.
Lehr HA, Hubner C, Finckh B, et al. Role of leukotrienes in leukocyte adhesion following systemic administration of oxidatively modified human low density lipoprotein in hamsters. J Clin Invest. 1991;88:9–14.
Kamler M, Lehr HA, Barker JH, et al. Impact of ischemia on tissue oxygenation and wound healing: intravital microscopic studies on the hairless mouse ear model. Eur Surg Res. 1993;25:30–7.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2015 Springer-Verlag London
About this chapter
Cite this chapter
Ozer, K. (2015). Mouse Cremaster Muscle Allograft Model. In: Siemionow, M. (eds) Plastic and Reconstructive Surgery. Springer, London. https://doi.org/10.1007/978-1-4471-6335-0_16
Download citation
DOI: https://doi.org/10.1007/978-1-4471-6335-0_16
Published:
Publisher Name: Springer, London
Print ISBN: 978-1-4471-6334-3
Online ISBN: 978-1-4471-6335-0
eBook Packages: MedicineMedicine (R0)