How to Perform 3D Histology on Different Types of Tumors

  • Helmut Breuninger
  • Patrick Adam


3D histology is intended for tumors at difficult sites where a tissue-saving surgical technique is needed or if local R0 resection must be ensured due to the aggressive growth of the tumor (Table 8.1) [1]. Precondition for this technique is a continuous local, clinically invisible infiltration of the tumor. Therefore, the margins of the excised tumor have to be investigated histologically in order to verify total removal of the tumor. For safety reasons, it is recommendable to increase the width of the safety margins in the primary excision of the tumor relative to the overall size of the tumor. Only in more distinguished locations with important structures or for aesthetical reasons, it is recommendable to choose smaller safety margins in order to save healthy tissue. However, it is imperative that in any case, the full removal is diagnosed by the presence of histologically clear margins.


Basal Cell Carcinoma Safety Margin Merkel Cell Carcinoma Primary Excision Desmoplastic Melanoma 
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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Helmut Breuninger
    • 1
  • Patrick Adam
    • 2
  1. 1.Universitätsklinikum Tübingen HautklinikTübingenGermany
  2. 2.Pathologie und Pathologische AnatomieUniversitätsklinikum TübingenTübingenGermany

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