Neuroprotective Mechanisms of Intermittent Hypoxia: An In Vitro Study

  • Galina SkiboEmail author
  • Maxim Orlovsky
  • Anastasiia Maistrenko
  • Victor Dosenko
  • Iryna Lushnikova


It is well known that brief sublethal anoxia episodes can render neurons resistant against subsequent longer or more severe anoxia and ischemia. The progress in applications of hypoxia in medicine is largely dependent on availability of appropriate experimental models. In this chapter, we describe an in vitro model for anoxia investigations using organotypic-cultured hippocampal slices. We investigate the efficiency of different regimes of anoxia preconditioning. We also describe a new single-cell real-time RT-PCR method for analyzing individual neurons within cultured hippocampal slices. This approach allows investigation of short-term neuronal reactions on anoxia preconditioning and oxygen-glucose deprivation – an experimental model of ischemic brain damage. Using these methods, we demonstrated that hypoxia-inducible factors (HIF), a family of transcription regulators, are involved in the mechanisms of anoxia-induced neuroprotection in hippocampus. In particular, brief intermittent anoxia prevented the decrease in HIF-1 mRNA expression caused by severe oxygen-glucose deprivation, which is closely correlated with its neuroprotective action. Possible mechanisms of such endogenous neuroprotective effect are discussed.


Intermittent Hypoxia Hypoxic Precondition Organotypic Hippocampal Slice Culture Significant Neuronal Loss Anoxia Exposure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Anoxia preconditioning


Hanks’ balanced salt solution


Hypoxia-inducible factor


Oxygen-glucose deprivation


Propidium iodide



This work was funded by the grant of SFFD 46.2/001.


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Copyright information

© Springer-Verlag London 2012

Authors and Affiliations

  • Galina Skibo
    • 1
    Email author
  • Maxim Orlovsky
    • 1
  • Anastasiia Maistrenko
    • 1
  • Victor Dosenko
    • 2
  • Iryna Lushnikova
    • 1
  1. 1.Department of CytologyState Key Laboratory of Molecular and Cellular Biology, Bogomoletz Institute of Physiology, National Academy of SciencesKievUkraine
  2. 2.Department of General PathophysiologyState Key Laboratory of Molecular and Cellular Biology, Bogomoletz Institute of Physiology, National Academy of SciencesKievUkraine

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