Blueberry in Calcium- and Vitamin D-Enriched Fermented Milk Is Able to Modulate Bone Metabolism in Postmenopausal Women

  • Marie-Jeanne Davicco
  • Caroline Puel
  • Patrice Lebecque
  • Véronique CoxamEmail author


Research in the field of nutrition allows considering the establishment of a real prevention of osteoporosis. The value of fruits is discussed. Indeed, red fruits are particularly interesting for their high content in anthocyans, endowed with antioxidant and anti-inflammatory properties.

Fifty-six postmenopausal women (less than 6 years) aged 50–65 years, without HRT, were included in a controlled, randomized, double-blind placebo, prospective study, after a medical examination and a blood test. Throughout the 3-month study period, they kept their eating habits, limiting however consumption of red fruits. They were randomized into two groups of 28 subjects receiving either 0 or 120 mg of anthocyans daily, from blueberry extract. Those polyphenols were provided at the dose of 0 or 60 mg of active molecule in 100 ml of fermented milk (two bottles of 100 ml/day covering 25 and 20 % RDA for calcium and vitamin D, respectively).

Consumption of the milk enriched in polyphenols significantly improved serum bALP activity (an osteoblastic marker), without significant modification of CTX, a marker for bone resorption. This favorable orientation of bone metabolism could be explained by the contribution of anthocyans, the only noticeable difference between the two test foods. This finding is independent of the initial calcium and vitamin D consumption.

In conclusion, consumption of fermented milk enriched with calcium and ­vitamin D, containing blueberry, for 3 months, has corrected the insufficiency of vitamin D of postmenopausal women and resulted in improved bone formation, as indicated by the rise of a biomarker of osteoblastic activity. This benefit is probably related to the presence of blueberries (rich in polyphenols and phenolic acids).


Postmenopausal women Blueberry extract Bone biomarkers Calcium- and vitamin D-enriched milk 



Bone alkaline ­phosphatase


C-reactive protein


C-telopeptide cross-links




International unit


25-hydroxyvitamin D




Recommended dietary allowance


Standard error to the mean


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Copyright information

© Springer-Verlag London 2013

Authors and Affiliations

  • Marie-Jeanne Davicco
    • 1
    • 2
  • Caroline Puel
    • 1
    • 2
    • 3
  • Patrice Lebecque
    • 1
    • 2
  • Véronique Coxam
    • 1
    • 2
    Email author
  1. 1.Department of Human NutritionINRA, UMR1019, UNH, CRNH AuvergneClermont-FerrandFrance
  2. 2.Clermont Université, Université d’Auvergne, Unité de Nutrition HumaineClermont-FerrandFrance
  3. 3.Thématique NutritionSOREDAB, La Boissière EcoleParisFrance

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