Applications of Flow Cytometry in Bacterial Ecology
Monitoring activity and enumeration of bacteria in natural environments has always posed considerable problems. Conventional plating techniques have normally been employed but have increasingly been shown to be of limited value (Mills and Bell 1986) because only a small proportion of indigenous species (c. 1%) can be isolated by anyone technique (Jones 1977; Pickup 1991). The proposal that some bacteria can adopt a viable but non-culturable state has further complicated the recovery of bacteria from natural environments (Roszak and Colwell 1987). In an attempt to circumvent these difficulties methods have been devised for the direct microscopic enumeration of bacteria; for example, acridine orange staining which stains live bacteria green while debris (and presumably dead cells) appears orange to red. These methods, however, have a number of drawbacks that make them unreliable (Postma and Altemuller 1990; Page and Burns 1991). The requirement for accurate and rapid methods for detecting target species present within diverse and active populations has never been greater.
KeywordsFlow Cytometry Pyridine Nucleotide Fluorescein Diacetate Acridine Orange Staining Fluorescence Distribution
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