Abstract
The use of flow cytometry has revolutionized the study of immunology such that it is difficult now to envisage a modern laboratory without access to a cytometer. Other disciplines, such as haematology, are catching up. In contrast the technique has had limited impact on. microbiology and most microbiological studies, to date, have been with bacteria (Shapiro 1990). The question: What advantages does the adoption of expensive technology offer and what are the shortcomings of conventional microbiological assays? We will address this issue with reference to testing the efficacy of antifungal agents against Candida albicans.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Brajtburg JK, Price HD, Medoff G, Schlessinger D, Kobayashi GS (1974) The molecular basis for the selective toxicity of amphotericin B for yeast and filipin for animal oils. Antimicrob Agents Chemother 5:377–382
Brajtburg JK, Powderiey WG, Kobayashi GS, Medoff G (1990) Amphotericin B: current understanding of mechanisms of action. Antimicrob Agents Chemother 34:183–188
Butt TM, Hoch HC, Staples RC, St. Leger RJ (1989) Use of fluorochromes in the study of fungal cytology and differentiation. Exp Myco 13:303–320
Coleman K, Hunter PA, Ridgway Watt P (1983) A novel multichannel biophotometer and its use in determining antibiotic effects. In: Assessment of antimicrobial activity and resistance. Academic Press, London and New York
Gallis HA, Drew RH, Pickard WW (1990) Amphotericin B: 30 years of clinical experience. Rev Infect Dis 12:308–329
Halachmi D, Eilam Y (1989) Cystosolic and vacuolar Ca2+ concentrations in yeast measured with the Ca2+ sensitive fluorescence dye Indo-I. FEBS Lett 256:55–61
Jullien S, Capuozzo E, Salerno C, Crifo C (1991) Effects of polyene macrolides on the membrane potential of resting and activated human leukocytes. Biochem Int 24:307–319
Kukuruga M, Lynch M, Nakeff A, Sobel J (1991) Analysis of proliferation and differentiation of Candida albicans in vitro by multivariate flow cytometry. Cytometry Suppl. 5, abstr 41, p 34
O’Gorman MRG, Hopfer RL (1991) Amphotericin B susceptibility testing of Candida species by flow cytometry. Cytometry 12:743–747
Scott-Pore R (1990) Antibiotic susceptibility testing of Candida albicans by flow cytometry. Curr Microbiol 20:323–328
Shapiro HM (1988) Parameters and probes. In: Practical flow cytometry. Alan R Liss, New York, pp 115–198
Shapiro HM (1990) Flow cytometry in laboratory microbiology: new directions. ASM News Lett 584, November
Sokol-Anderson ML, Brajtburg J, Medoff G (1986) Amphotericin B-induced oxidative damage and killing of Candida albicans J Infect Dis 154:76–83
Wilson HA, Chused T (1985) Lymphocyte membrane potential and Ca2+ sensitive potassium channels described by oxonol dye fluorescence measurements. J Cell Physiol 125:72–81
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1993 Springer-Verlag London
About this chapter
Cite this chapter
Carter, E.A., Paul, F.E., Hunter, P.A. (1993). Cytometric Evaluation of Antifungal Agents. In: Lloyd, D. (eds) Flow Cytometry in Microbiology. Springer, London. https://doi.org/10.1007/978-1-4471-2017-9_8
Download citation
DOI: https://doi.org/10.1007/978-1-4471-2017-9_8
Publisher Name: Springer, London
Print ISBN: 978-1-4471-2019-3
Online ISBN: 978-1-4471-2017-9
eBook Packages: Springer Book Archive