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The Physical and Biological Basis for Flow Cytometry of Escherichia coli

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Flow Cytometry in Microbiology

Abstract

Flow cytometry is a method for measuring the fluorescence and light scattering of individual cells in large numbers. By labelling the cells with fluorescent molecules that bind with high specificity to one particular cellular constituent it is possible to measure the content of the constituent. Such a fluorescent tag may be either a dye molecule with a high binding specificity for the particular component to be measured or a fluorescence conjugated antibody. The light scattering of the cells gives information on their size and to some extent also on shape and structure (Salzman et al. 1979, 1990).

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Authors
  1. Erik Boye
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  2. Harald B. Steen
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Editor information

Editors and Affiliations

  1. University of Wales College of Cardiff, PO Box 915, Cardiff, CF1 3TL, Wales

    David Lloyd BSc, PhD, DSc (Professor of Microbiology) (Professor of Microbiology)

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© 1993 Springer-Verlag London

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Boye, E., Steen, H.B. (1993). The Physical and Biological Basis for Flow Cytometry of Escherichia coli . In: Lloyd, D. (eds) Flow Cytometry in Microbiology. Springer, London. https://doi.org/10.1007/978-1-4471-2017-9_2

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  • DOI: https://doi.org/10.1007/978-1-4471-2017-9_2

  • Publisher Name: Springer, London

  • Print ISBN: 978-1-4471-2019-3

  • Online ISBN: 978-1-4471-2017-9

  • eBook Packages: Springer Book Archive

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