Rapid Analysis of Microorganisms Using Flow Cytometry
A consistent limitation to the implementation of proactive product quality management has been the delay associated with microbiological analysis. Although standard microbiological techniques allow the detection of single bacteria, amplification of the signal is required through growth of a single cell into a colony on a plate. This process can be relatively time-consuming. For example, where an organism has a mean doubling time of approximately 30 min, the development of a colony containing 106 organisms (i.e. visible to the naked eye) will take between 18 and 24 h. In the case of yeast, a common spoilage organism, this period will be considerably longer, taking 3–7 days depending upon the yeast strain. In the case of final product testing, especially for products with a short shelf-life, microbiological data may not therefore be available until several days after the product has been released to the market. This can lead to product recall, with associated cost implications and reduction in customer confidence.
KeywordsBiomass Fermentation Filtration Chrome Europe
Unable to display preview. Download preview PDF.
- Bankes P, Rowe D, Betts RP (Campden Food and Drink Research Association, UK) (1991) The rapid detection of yeast spoilage using the ChemFlow System. Technical memorandum no. 621, May 1991Google Scholar
- Cady P (1975) Rapid automated bacterial identification by impedance measurements. In: Heden CG, Illeni T (eds) New approaches to the identification of micro-organisms. Wiley, Chichester, pp 74–99Google Scholar
- Chevilbtte L, Laplace-Builhe C, Louvel L, Van Hoegaerden M, Theilleux J (1990) Application of flow cytometry to industrial microbiology. In: 5th European Congress on Biotechnology, Copenhagen, p 304 (abstr)Google Scholar
- Desnouveaux R, Lecomte C, De Colombel E et al. (1990) Use of flow cytometry for yeast and mould detection in process control of fermented milk products: the ChemFlow system. A factory study. Biotech Forum Europe 3:224–229Google Scholar
- Easter MC, Gibson DM (1989) Detection of micro-organisms by electrical methods. Progr Ind Microbiol 26:57–199Google Scholar
- Gatley S (1989) Rapid process control with flow cytometry. Food Technology InternationalEuropeGoogle Scholar
- Gatley S (1990) Digital microbiology: a radical approach to the design and development of a new rapid microbiology system. Biotech Forum Europe 6:478–482Google Scholar