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Flow Cytometry in Microbiology pp 131–142Cite as

Analysis of Microalgae and Cyanobacteria by Flow Cytometry

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Abstract

Flow cytometers first became available commercially around 1970, and early studies of algal cultures were carried out by Paau et al. (1978), Price et al. (1978) and Trask et al. (1982). A workshop report by Yentsch et al. (1983b) drew attention to the remarkable power of flow cytometry for aquatic particle analysis, and this was followed by the rapid adoption of the technique in oceanographic research. A special volume of Cytometry (Yentsch and Horan 1989) has been devoted to this topic, and the proceedings of a lively NATO Advanced Study Institute have recently been published (Demers 1992).

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References

  • Balfoort HW, Snoek J, Smits JRM, Breedveld LW, Hofstraat JW, Ringelberg J (1992) Automatic identification of algae: neural network analysis of flow cytometric data. J Plankton Res (in press)

    Google Scholar 

  • Berglund D, Eversman S (1988) Flow cytometric measurement of pollutant stresses on algal cells. Cytometry 9:150–155

    Article  PubMed  CAS  Google Scholar 

  • Bonaly J, Bre MH, Lefort-Tran M, Mestre JC (1987) A flow cytometric study of DNA staining in situ in exponentially growing and stationary Euglena gracilis. Cytometry 8:42–45

    Article  PubMed  CAS  Google Scholar 

  • Buonnacorsi GA, Cunningham A (1990) Azimuthal inhomogeneity in the forward light scattered from microalgal colonies, and its use as a morphological indicator in flow cytometry. Limnol Oceanogr 35: 1170–1175

    Article  Google Scholar 

  • Burkill PH (1987) Analytical flow cytometry and its application to marine microbial ecology. In: Sleigh MA (ed) Microbes in the sea. Ellis Horwood, Chichester, pp 139–166

    Google Scholar 

  • Burkill PH, Mantoura RFC (1990) The rapid analysis of single marine cells by flow cytometry. Phil Trans R Soc Lond A 333:49–61

    Article  Google Scholar 

  • Bllirsheim KY, Harboe T, Johnsen T, Norland S, Nygaard K (1989) Flow cytometric characterisation and enumeration of Chrysochromulina polylepis during a bloom along the Norwegian coast. Mar Ecol Prog Ser 54:307–309

    Article  Google Scholar 

  • Campbell L, Carpenter EJ (1987) Characterization of phycoerythrin-containing Synechococcus spp. populations by immunofluorescence. J Plankton Res 9:1167–1181

    Article  Google Scholar 

  • Chisholm SW, Olson RJ, Zettler ER, Goericke R, Waterbury JB, Welschmeyer NA (1988) A novel free-living prochlorophyte abundant in the oceanic euphotic zone. Nature 334:340–343

    Article  Google Scholar 

  • Chisholm SW, Armbrust EV, Olson RJ (1986) The individual cell in phytoplankton ecology: cell cycles and applications of flow cytometry. Can Bull Fish Aquat Sci 214:343–369

    Google Scholar 

  • Cooksey KE, Guckert JB, Williams SA, Callis PR (1987) Fluorimetric determination of the neutral lipid content of micro algal cells using nile red. J Microbiol Meth 6:333–345

    Article  CAS  Google Scholar 

  • Cucci TL, Shumway SE, Newell RC, Selvin R, Guillard RL, Yentsch CM (1985) Flow cytometry: a new method for characterization of differential ingestion, digestion and egestion by suspension feeders. Mar Ecol Prog Ser 24:201–204

    Article  Google Scholar 

  • Cucci TL, Shumway SE, Brown WS, Newell CR (1989) Using phytoplankton and flow cytometry to analyze grazing by marine organisms. Cytometry 10:658–669

    Article  Google Scholar 

  • Cunningham A (1990) A low cost, portable flow cytometer specifically designed for phytoplankton analysis. J Plankton Res 12:149–160

    Article  Google Scholar 

  • Cunningham A (1991) Fluorescence pulse shape as a morphological indicator in the analysis of colonial microalgae by flow cytometry. J Microbiol Meth 11:27–36

    Article  CAS  Google Scholar 

  • Cunningham A, Buonnacorsi GA (1992) Narrow-angle forward light scattering from individual algal cells: implications for size and shape discrimination in flow cytometry. J Plankton Res 14:223–234

    Article  Google Scholar 

  • Cunningham A, Leftley JW (1986) Application of flow cytometry to algal physiology and phytoplankton ecology. FEMS Microbiol Rev 32:159–164

    Article  CAS  Google Scholar 

  • Darzynkiewicz Z, Crissman HA (1990) Flow cytometry. Methods in cell biology 33. Academic Press, New York

    Google Scholar 

  • Demers S (ed) (1991) Particle analysis in oceanography: proceedings of a NATO Advanced Study Institute at Acquefreda di Maratea, Italy, November 1990. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Dorsey J, Yentsch CM, Mayo S, McKenna C (1989) Rapid analytical technique for the assessment of cell metabolic activity in marine microalgae. Cytometry 10:622–628

    Article  PubMed  CAS  Google Scholar 

  • Dubelaar GBJ, Visser JWM, Donze M (1987) Anomalous behaviour of forward and perpendicular light scattering of a cyanobacterium owing to intracellular gas vacuoles. Cytometry 8:405–412

    Article  PubMed  CAS  Google Scholar 

  • Dubelaar GBJ, Groenewegen AC, Stokdijk W, van den Engh GJ, Visser JWM (1989) Optical plankton analyser: a flow cytometer for plankton analysis. II. Specifications. Cytometry 10:529–539

    Article  PubMed  CAS  Google Scholar 

  • Frankel DS, Olson RJ, Frankel SL, Chisholm SW (1989) Use of a neural net computer system for analysis of flow cytometric data of phytoplankton populations. Cytometry 10:540–550

    Article  PubMed  CAS  Google Scholar 

  • Gerritsen J, Sanders RW, Bradley SW, Porter K (1987) Individual feeding variability of protozoan and crustacean zooplankton analyzed with flow cytometry. Limnol Oceanogr 32:691–699

    Article  Google Scholar 

  • Heiden T, Göhde W, Tribukait B (1990) Two-wavelength mercury arc lamp excitation for flow cytometric DNA-protein analyses. Anticancer Res 10: 1555–1562

    PubMed  CAS  Google Scholar 

  • Hilton J, Rigg E, Jaworski G (1988) In vivo algal fluorescence, spectral change due to light intensity changes and the automatic characterisation of algae. Freshwater Bioi 20:375–382

    Article  Google Scholar 

  • Hilton, J, Rigg E, Jaworski G (1989) Algal identification using in vivo fluorescence spectra. J Plankton Res 11:65–74

    Article  Google Scholar 

  • Hofstraat JW, van Zeijl WJM, Peeters JCH, Peperzak L, Dubelaar GBJ (1990) Flow cytometry and other optical methods for characterization and quantification of phytoplankton in seawater. SPIE 1269: Environment and Pollution Measurement Sensors and Systems, pp 116–132

    Google Scholar 

  • Hutter KJ, Eipel HE (1979) Microbial determinations by flow cytometry. J Gen Microbiol 113:369–375

    Article  PubMed  CAS  Google Scholar 

  • Hutter KJ, Eipel HE, Stohr M (1980) Flow cytometric analysis of microbial cell constituents after heavy metal intoxication. Acta Pathol Microbiol Scand (Suppl. 274):317–321

    Google Scholar 

  • Lefort-Tran M, Bre MH, Pouphile M, Manigault P (1987) DNA flow cytometry of control Euglena and cell cycle blockade of vitamin B12 starved cells. Cytometry 8:46–54

    Article  PubMed  CAS  Google Scholar 

  • Li WKW, Wood AM (1988) Vertical distribution of North Atlantic ultraphytoplankton: analysis by flow cytometry and epifluorescence microscopy. Deep Sea Res 35:1615–1638

    Article  Google Scholar 

  • Li WKW, Lewis MR, Lister A (1991) Picoplankton in the Gulf of Policastro. Signal and noise. Bigelow Laboratory Specialist Publication 4, part 2, p 3

    Google Scholar 

  • Olson RJ, Frankel SL, Chisolm SW, Shapiro HM (1983) An inexpensive flow cytometer for the analysis of fluorescence signals in phytoplankton: chlorophyll and DNA distributions. J Exp Marine Bioi Ecol 68:1–16

    Article  Google Scholar 

  • Olson RJ, Zettler ER, Anderson OK (1989) Discrimination of eukaryotic phytoplankton cell types from light scatter and autofluorescence properties measured by flow cytometry. Cytometry 10:636–643

    Article  PubMed  CAS  Google Scholar 

  • Olson RJ, Chisholm SW, Zcttlcr ER, Altabet MA, Duscnbcrry J A (1990) Spatial and temporal distributions of prochlorophyte picoplankton in thc North Atlantic Occan. Dccp Sca Rcs 37:1033–1051

    Google Scholar 

  • Ormerod MG (cd) (1990) Flow cytomctry: a practical approach. IRL Press, Oxford

    Google Scholar 

  • Paau AS, Oro J, Cowlcs JR (1978) Application of flow microfluoromctry to thc study of algal cclls and isolatcd chloroplasts. J Exp Bot 29: 1011–1020

    Article  CAS  Google Scholar 

  • Pecters JCH, Dubclaar GBJ, Ringelberg J, Visser JWM (1989) Optical plankton analyser: a llow cytometcr for plankton analysis. I. Design considerations. Cytometry 10:522–528

    Article  Google Scholar 

  • Prezelin BB, Boczar BA (1986) Molecular bases of cell absorption and lluorescence in phytoplankton: potential applications to studies in optical oceanography. In: Round FE, Chapman DJ (eds) Progr Phycol Res 4:349–464

    Google Scholar 

  • Pricc BJ, Kollman VH, Salzman GC (1978) Light scatter analysis of microalgac: correlation of scatter patterns from pure and mixed asynchronous cultures. Biophys J 22:29–36

    Article  Google Scholar 

  • Samson G, Popovic R (1988) Usc of algal fluorescence for determination of phytotoxicity of heavy metals and pesticides as environmental pollutants. Ecotoxicol Environ Safety 16:272–278

    Article  PubMed  CAS  Google Scholar 

  • Shapiro HM (1988) Practical flow cytometry, 2nd edn. Liss-Wiley, New York

    Google Scholar 

  • Steen HB (1986) Simultaneous separate detection of low angle and large angle light scattering in an arc lamp-based flow cytometer. Cytometry 7:445–449

    Article  PubMed  CAS  Google Scholar 

  • Trask BJ, van den Engh GJ, Elgershuizen JHBW (1982) Analysis of phytoplankton by flow cytometry. Cytometry 2:258–264

    Article  PubMed  CAS  Google Scholar 

  • van Dilla MA, Dean PN, Laerum OD, Melamed MR (1985) Flow cytometry: Instrumentation and data analysis. Academic Press, New York

    Google Scholar 

  • van de Hulst HC (1957) Light scattering by small particles. Wiley, New York

    Google Scholar 

  • Vaulot D, Olson RJ, Chisholm SW (1986) Light and dark control of the cell cycle in two phytoplankton species. Exp Cell Res 167:38–52

    Article  PubMed  CAS  Google Scholar 

  • Yentsch CM (1981) Flow cytometric analysis of cellular saxitoxin in the dinoflagellate Gonyaulax lamarensis var excavala. Toxicon 19:611–621

    Article  PubMed  CAS  Google Scholar 

  • Yentsch CM (1990) Environmental health: flow cytometric mcthods to assess our watcr world. In: Darzynkiewicz Z, Crissman HA (eds) Flow cytometry. Academic Press, New York, pp 575–612

    Chapter  Google Scholar 

  • Yentsch CM, Horan PK (eds) (1989) Cytometry in aquatic sciences. Cytometry 10:497–669

    Google Scholar 

  • Yentsch CS, Yentsch CM (1979) Fluorescence spectral signatures: the charactcrization of phytoplankton populations by the use of excitation and emission spectra. J Marine Res 37:471–483

    CAS  Google Scholar 

  • Yentsch CM, Mague FM, Horan PK and Muirhead K (1983a) Flow cytometric dcterminations on individual cells of the dinoflagellate Gonyaulax tamarensis var excavala. J Exp Marine Bioi EcoI 67:175–183

    Article  CAS  Google Scholar 

  • Yentsch CM, Horan PK, Muirhead K et al. (1983b) Flow cytometry and sorting: a technique for analysis and sorting of aquatic particles. Limnol Oceanogr 28: 1275–1280

    Article  Google Scholar 

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Authors
  1. Alex Cunningham
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Editors and Affiliations

  1. University of Wales College of Cardiff, PO Box 915, Cardiff, CF1 3TL, Wales

    David Lloyd BSc, PhD, DSc (Professor of Microbiology) (Professor of Microbiology)

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© 1993 Springer-Verlag London

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Cunningham, A. (1993). Analysis of Microalgae and Cyanobacteria by Flow Cytometry. In: Lloyd, D. (eds) Flow Cytometry in Microbiology. Springer, London. https://doi.org/10.1007/978-1-4471-2017-9_10

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  • DOI: https://doi.org/10.1007/978-1-4471-2017-9_10

  • Publisher Name: Springer, London

  • Print ISBN: 978-1-4471-2019-3

  • Online ISBN: 978-1-4471-2017-9

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