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Assaying of Oxalate in Plasma

  • G. P. Kasidas
Part of the The Bloomsbury Series in Clinical Science book series (BLOOMSBURY)

Abstract

Until recently the reliable measurement of oxalate in plasma presented considerable problems. Primarily, this was due to the extremely low level of the anion in plasma. The range of oxalate levels reported in normal plasma varied enormously (see Table 4.1). Such wide variations in the levels of oxalate in plasma probably arose from the difficulty in developing highly sensitive and specific assays to detect the low level of oxalate in plasma and also from the rapid in vitro oxalogenesis from glyoxylate (Akcay and Rose 1980), ascorbate (Kasidas and Rose 1986) and other precursors (France et al. 1985) during the storage and processing of samples. The problem of oxalogenesis from ascorbate in urine was discussed in Chapter 2. In plasma, because of the neutral/near-alkaline pH environment and the relatively higher concentration ratio of ascorbate to oxalate which is normal in this matrix, oxalogenesis from ascorbate may pose even greater problems (see below). In vivo radioisotope assays (Williams et al. 1971; Pinto et al. 1974; Hodgkinson and Wilkinson 1974; Constable et al. 1979; Prenen et al. 1982) have consistently yielded plasma oxalate levels in normal subjects of 0.4−2.8 μmol/l whereas the earher in vitro assays (see Table 4.1) produced values ranging from 30 to 300 μmol/1. The gap between the two approaches for determining plasma oxalate has narrowed considerably as a result of recognition and subsequent correction of the problems arising from spontaneous in vitro oxalogenesis and the development of highly specific and sensitive assays.

Keywords

High Performance Liquid Chromatographic Oxalic Acid Urinary Oxalate Oxalate Oxidase Primary Hyperoxaluria 
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References

  1. Ahmad S, Hatch M (1985) Hyperoxalaemia in renal failure and the role of hemoperfusion and hemodialysis in primary oxalosis. Nephron 41: 235–240PubMedCrossRefGoogle Scholar
  2. Akcay T, Rose GA (1980) The real and apparent plasma oxalate. Clin Chim Acta 101: 305–311PubMedCrossRefGoogle Scholar
  3. Barber HH, Gallimore EJ (1940) The metabolism of oxalic acid in the animal body. Biochem J 34: 144–148PubMedGoogle Scholar
  4. Barrett JFB (1943) The oxalate content of blood. Biochem J 37: 254–256PubMedGoogle Scholar
  5. Bennett DJ, Cole FE, Frohlich ED, Erwin DT (1979) A radioenzymatic isotope-dilution assay for oxalate in serum or plasma. Clin Chem 25: 1810–1813PubMedGoogle Scholar
  6. Boer P, Van Leersum L, Endeman HJ (1984) Determination of plasma oxalate with oxalate oxidase. Clin Chim Acta 137: 53–60PubMedCrossRefGoogle Scholar
  7. Borland WW, Payton CD, Simpson K, MacDougall AI (1987) Serum oxalate in chronic renal failure. Nephron 45: 119–121PubMedCrossRefGoogle Scholar
  8. Carr PW, Bowers LD (1980) Principles of kinetic and equilibrium methods of analysis. In: Elving PJ, Winefordner JD, Kolthoff IM (eds) Immobilised enzymes in analytical and clinical chemistry. John Wiley & Sons, New York, pp 95–147Google Scholar
  9. Chambers MM, Russell JC (1973) A specific assay for plasma oxalate. Clin Biochem 6: 22–28PubMedCrossRefGoogle Scholar
  10. Cole FE, Gladden KM, Bennett DJ, Erwin DT (1984) Human plasma oxalate concentration reexamined. Clin Chim Acta 139: 137–143PubMedCrossRefGoogle Scholar
  11. Constable AR, Joekes AM, Kasidas GP, O’Reagan P, Rose GA (1979) Plasma level and renal clearance of oxalate in normal subjects and in patients with primary hyperoxaluria or chronic renal failure or both. Clin Sci 56: 229–304Google Scholar
  12. Crawhall JC, Watts RWE (1961) The oxalate content of human plasma. Clin Sci 20: 357–366PubMedGoogle Scholar
  13. Endo R (1969) Rise and fall of oxalic acid in blood urine II Normal values. Med J Mutual Aid Assoc (Tokyo) 18: 254–256Google Scholar
  14. Finch AM, Kasidas GP, Rose GA (1981) Urine composition in normal subjects after oral ingestion of oxalate rich foods. Clin Sci 60: 411–418PubMedGoogle Scholar
  15. Flaschenträger B, Muller PB (1938) Zur biologic der Oxalsäure 1. Microbestimmung der Oxalsäure im Harn und Blut. Z Physiol Chem 251: 52–77CrossRefGoogle Scholar
  16. France NC, Windleborn EA, Wallace MR (1985) In vitro oxalogenesis and measurement of oxalate in serum. Clin Chem 31: 335–336PubMedGoogle Scholar
  17. Gelot MA, Lavoue G, Belleville F, Nabet P (1980) Determination of oxalate in plasma and urine gas chromatography. Clin Chim Acta 106: 279–285PubMedCrossRefGoogle Scholar
  18. Greger R, Lang F, Oberleithner H, Deetjen P (1978) Handling of oxalate by the rat kidney. Pflugers Arch 374: 243–248PubMedCrossRefGoogle Scholar
  19. Hallson PC, Rose GA (1974) A simplified and rapid enzymatic method for determination of urinary oxalate. Clin Chim Acta 55: 29–39PubMedCrossRefGoogle Scholar
  20. Hargreave TB, Sali A, MacKay C, Sullivan M (1977) Diurnal variation in urinary oxalate. Br J Urol 49: 597–600PubMedCrossRefGoogle Scholar
  21. Hatch M, Bourke E, Costello J (1977) New enzymatic method for serum oxalate determination. Clin Chem 23: 76–78PubMedGoogle Scholar
  22. Hodgkinson A, Wilkinson R (1974) Plasma oxalate concentration and renal excretion of oxalate in man. Clin Sci Molec Med 46: 61–73Google Scholar
  23. Ichiyama A, Nakai E, Funai T, Oda T, Katafuchi R (1985) Spectrophotometric determination of oxalate in urine and plasma with oxalate oxidase. J Biochem (Tokyo) 98: 1375–1385Google Scholar
  24. Jerez E (1986) Determination de acido oxálico en plasma por chromatografia liquid de atla efficacia. Rev Esp Fisiol 42: 441–448PubMedGoogle Scholar
  25. Kasidas GP, Rose GA (1980) Oxalate content of some common foods; determination by an enzymatic method. J Human Nutr 35: 255–266Google Scholar
  26. Kasidas GP, Rose GA (1986) Measurement of plasma oxalate in healthy subjects and in patients with chronic renal failure using immobilised oxalate oxidase. Clin Chim Acta 154: 49–58PubMedCrossRefGoogle Scholar
  27. Kasidas GP, Rose GA (1987) Measurement of plasma oxalate and when this is useful. In: Gasser G, Vahlensieck W (eds) Pathogenese und Klinik der Harnsteine XII. Steinkopff, Darmstadt, pp 143–147CrossRefGoogle Scholar
  28. Knight TF, Senekjian HO, Weinman EJ (1979) Effect of para-aminohippurate on renal transport of oxalate. Kidney Int 15: 38–42PubMedCrossRefGoogle Scholar
  29. Knowles CF, Hodgkinson A (1972) Automated enzymatic determination of oxalic acid in human serum. Analyst 97: 474–481CrossRefGoogle Scholar
  30. Kohlbecker G (1981) Direct spectrophotometric determination of serum and urinary oxalate with oxalate oxidase. J Clin Chem Clin Biochem 19: 1103–1106PubMedGoogle Scholar
  31. Krugers Dagneaux PGLC, Elhorst JTK, Olthuis FMFG (1976) Oxalic acid determination in plasma. Clin Chim Acta 71: 319–325PubMedCrossRefGoogle Scholar
  32. Lopez M, Tuchman M, Scheinman JI (1985) Capillary gas chromatography measurement of oxalate in plasma and urine. Kidney Int 28: 82–84PubMedCrossRefGoogle Scholar
  33. Macaluso MP, Berg NO (1959) Calcium oxalate crystals in the kidney in acute tubular necrosis and other renal diseases with functional failure. Arch Path Microbiol Scand 46: 197–205CrossRefGoogle Scholar
  34. Merz KW, Maugeri S (1931) Über das Vorkommen and die Bestimmung der Oxalsäure im Blut. Physiol Chem 201: 31–37CrossRefGoogle Scholar
  35. Nuret P, Offner M (1978) A new method for determination of oxalate in blood serum by gas chromatography. Clin Chim Acta 82: 9–12PubMedCrossRefGoogle Scholar
  36. Osswald H, Hautmann R (1979) Renal elimination kinetics and plasma half life of oxalate in man. Urol Int 34: 440–450PubMedCrossRefGoogle Scholar
  37. Parkinson IS, Kealey T, Laker MF (1985) The determination of plasma oxalate concentrations using an enzyme/bioluminescent assay. Clin Chim Acta 152: 335–345PubMedCrossRefGoogle Scholar
  38. Pernet JL, Pernet A (1965) Dosage colorimetrique de Tacide oxalique dans les millieux biologiques. Ann Biol Clin 23: 1189–1207Google Scholar
  39. Pinto B, Crespi G, Sole-Bacells F, Barcelo P (1974) Patterns of oxalate metabolism in recurrent stone formers. Kidney Int 5: 285–291PubMedCrossRefGoogle Scholar
  40. Prenen JAC, Boer P, Dorhout Mees EJ, Endeman HJ, Spoor SM, Oei HY (1982) Renal clearance of (14C) oxalate: comparison of constant infusion with single injection techniques. Clin Sci 63: 47–51PubMedGoogle Scholar
  41. O’Reagan PFB, Constable AR, Harrison AR, Joekes AM, Kasidas GP, Rose GA (1979) The management of the primary hyperoxaluric patient. In: Rose GA, Robertson WG, Watts RWE (eds) Oxalate in human biochemistry and clinical pathology. Wellcome Foundation, London, pp 209–219Google Scholar
  42. Ramsay AG, Reed RG (1984) Oxalate removal by hemodialysis in end stage renal disease. Am J Kidney Dis 4: 123–127PubMedGoogle Scholar
  43. Rehmert U, Wicher K, Ruge W, Bahlmann J (1983) Oxalate determination in plasma with oxalate oxidase. Investigation in healthy persons and patients with terminal renal failure before and after dialysis. Lab Med 7: 29–32Google Scholar
  44. Rose GA (1985) Advances in analysis of urinary oxalate; the ascorbate problem solved. In: Schwille PO, Smith LH, Robertson WG, Vahlensieck W (eds) Urolithiasis and related clinical research. Plenum Press, New York, pp 637–644Google Scholar
  45. Salyer WR, Keren D (1973) Oxalosis as a complication of chronic renal failure. Kidney Int 4: 61–66PubMedCrossRefGoogle Scholar
  46. Samsoondar J, Moore RW, Kellen JA (1983) Enzymatic determination of oxalates. Enzymes 30: 273–276Google Scholar
  47. Sugiura M, Yamamura H, Hirano K et al. (1980) Enzymatic determination of serum oxalate. Clin Chim Acta 105: 393–399PubMedCrossRefGoogle Scholar
  48. Suzuki S (1934) The oxalic acid in blood. Jpn J Med Sci Biochem 2: 291–303Google Scholar
  49. Thomsen A (1935) Über den oxalsäuregehalt des blutes. Hoppe-Seyler’s Z Physiol Chem 237: 199–213CrossRefGoogle Scholar
  50. Tiselius HG, Almgard LE (1977) The diurnal urinary excretion of oxalate and the effect of pyridoxine and ascorbate on oxalate excretion. Eur Urol 3: 41–46PubMedGoogle Scholar
  51. Tocco DJ, Duncan AFW, Noll RM, Duggan DE (1979) An electron capture procedure for the estimation of oxalic acid in urine. Anal Biochem 94: 470–476PubMedCrossRefGoogle Scholar
  52. Vahlensieck EW, Bach D, Hesse A (1982) Circadian rhythm of lithogenic substances in urine. Urol Res 10: 195–203PubMedGoogle Scholar
  53. Weinman EJ, Frankfurt SJ, Ince A, Sansom S (1978) Renal tubular transport of organic acids. J Clin Invest 61: 801–806PubMedCrossRefGoogle Scholar
  54. Williams HE, Johnson GA, Smith LH (1971) The renal clearance of oxalate in normal subjects and in patients with primary hyperoxaluria. Clin Sci 41: 213–218PubMedGoogle Scholar
  55. Wolthers BG, Hayer M (1982) The determination of oxalic acid in plasma and urine by means of capillary gas chromatography. Clin Chim Acta 120: 87–102PubMedCrossRefGoogle Scholar
  56. Zarembski PM, Hodgkinson A (1963) The renal clearance of oxalic acid in normal subjects and in patients with primary hyperoxlauria. Invest Urol 1: 87–93PubMedGoogle Scholar
  57. Zarembski PM, Hodgkinson A (1965) The fluorimetric determination of oxalic acid in blood and other biological materials. Biochem J 96: 717–721PubMedGoogle Scholar
  58. Zarembski PM, Hodgkinson A, Parsons FM (1966) Elevation of the concentration of plasma oxalic acid in renal failure. Nature 212: 511–512PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1988

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  • G. P. Kasidas

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