Some Functional Properties of Human Bronchoalveolar and Dispersed Lung Mast Cells

  • Kevin Charles Flint
Part of the The Bloomsbury Series in Clinical Science book series (BLOOMSBURY)

Abstract

Much of our knowledge of mast cell function stems from early work with rodent cells, usually those harvested from the rat peritoneal cavity. In the light of the now considerable evidence of heterogeneity of mast cell function between different species, this work must be interpreted with great caution. The challenge of human lung fragments via IgE-dependent mechanisms in vitro is one step closer to human disease. However, activation of lung fragments will include effects on vascular tissue and lung parenchyma in addition to bronchi. The activation of such a mixed population of cells produces results that are very variable (Church and Young 1983) and the contribution of any single cell type to the response of the intact tissue is impossible to define. Dispersion of free cells from lung fragments allows the study of the function of mast cells in isolated cell suspensions, and ultimately with the purification of mast cells, their function in isolation. However, with increasing evidence of heterogeneity of mast cell function even between mast cells from different sites in the same tissue (Pearce 1982), the response of mast cells dispersed from the lung parenchyma may not be relevant to events occurring at the mucosal surface. Thus mast cells in bronchoalveolar lavage may possess functional properties that are distinct from those in dispersed cell preparations. In direct comparisons between these two preparations differences and similarities are now being defined.

Keywords

Histamine Prednisolone Theophylline Mast Salbutamol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Borum P, Mygind N (1980) Inhibition of the immediate reaction in the nose by the beta-2 adrenostimulant fenoterol. J Allergy Clin Immunol 66: 25–32PubMedCrossRefGoogle Scholar
  2. Butchers PR, Fullarton JR, Skidmore IF, Thompson LE, Varday J, Wheeldon A (1979) A comparison of the anti-anaphylactic activities of salbutamol and disodium cromoglycate in the rat, the rat mast cell and in human lung tissue. Br J Pharmacol 67: 23–32PubMedGoogle Scholar
  3. Church MK (1978) Cromoglycate-like anti-allergic drugs: a review. Drugs Today 14: 281–341Google Scholar
  4. Church MK, Young KD (1983) The characteristics of histamine release from human lung fragments by sodium cromoglycate, salbutamol and chlorpromazine. Br J Pharmacol 78: 671–679PubMedGoogle Scholar
  5. Church MK, Holgate ST, Pao GJK (1983) Histamine release from mechanically and enzymatically dispersed human lung mast cells: inhibition by salbutamol and cromoglycate. Br J Pharmacol 79: 347pGoogle Scholar
  6. Flint KC, Leung KBP, Pearce FL, Hudspith BN, Brostoff J, Johnson NMcI (1985) Human mast cells recovered by bronchoalveolar lavage: their morphology, histamine release and the effects of sodium cromoglycate. Clin Sci 68: 427–432PubMedGoogle Scholar
  7. Johnson AR, Moran NC (1970) Inhibition of the release of histamine from rat mast cells: the effects of cold and adrenergic drugs on the release of histamine by 48/80 and antigen. J Pharmacol Exp Ther 175: 632–640PubMedGoogle Scholar
  8. Lal S, Malhotra S, Gribben S, Hodder D (1985) Nedocromil sodium: a new drug for the management of bronchial asthma. Thorax 39: 809–812CrossRefGoogle Scholar
  9. Lawrence EC, Blaese RM, Martin RR, Stevens PM (1978) Immunoglobulin secreting cells in normal human bronchial lavage fluids. J Clin Invest 62: 832–835PubMedCrossRefGoogle Scholar
  10. Law D, Jackson L, Fulmer J (1982) Bronchoalveolar lavage analysis in the interstitial lung diseases. Am Rev Resp Dis 125: 105–108Google Scholar
  11. Leung KBP, Flint KC, Brostoff J, Hudspith BN, Johnson NMcI, Pearce FL (1986) Some properties of mast cells obtained by human bronchoalveolar lavage. Agents and Actions 18: 100–112CrossRefGoogle Scholar
  12. Martin AL, Atkins PC, Dunsky PH, Zweiman B (1980) The effect of theophylline, terbutaline and prednisolone upon antigen-induced bronchospasm and mediator release. J Allergy Clin Immunol 68: 286–289Google Scholar
  13. Merrill WW, Naegel GP, Reynolds HY (1980) Analysis of normal human bronchoalveolar lavage fluid IgE and comparison to immunoglobulins G and A. J Lab Clin Med 94: 494–500Google Scholar
  14. Orr TSC (1977) Mode of action of disodium cromoglycate. Acta Allergologica (Suppl 13) 32: 9–27Google Scholar
  15. Pearce FL (1982) Functional heterogeneity of mast cells from different species and tissues. Klin Wschr 60: 954–957PubMedCrossRefGoogle Scholar
  16. Schulman ES, Kagey-Sabotka A, MacGlashan DW et al. (1983) Heterogeneity of human lung mast cells. J Immunol 131: 1936–1941PubMedGoogle Scholar
  17. Schumacker NJ (1980) Effect of a beta-adrenergic agonist, fenoterol, on nasal sensitivity to allergen. J Allergy Clin Immunol 66: 33–37CrossRefGoogle Scholar
  18. Silverman M, Connolly NM, Balfour-Lynn L, Godfrey S (1972) Long term trial of disodium cromoglycate and isoprenaline in children with asthma. Br Med J 3: 378–381PubMedCrossRefGoogle Scholar
  19. Stokes TC, Morley J (1981) Prospects for an oral intal. Br J Dis Chest 75: 1–14PubMedCrossRefGoogle Scholar
  20. Young KD, Church MK (1983) Passive anaphylaxis in human lung fragments as a model for testing anti-allergic drugs: its variability and constraints. Int Arch Allergy Appl ImmunolGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • Kevin Charles Flint
    • 1
  1. 1.Department of ImmunologyThe Middlesex Hospital Medical SchoolLondonUK

Personalised recommendations