The Use of Antibody Affinity Chromatography and Other Methods in the Study of Pregnancy-Associated Proteins

  • R. G. Sutcliffe
  • B. M. Kukulska
  • L. V. B. Nicholson
  • W. F. Paterson
Conference paper

Abstract

The search for new pregnancy associated proteins has involved many disciplines and included many enzymatic, hormonal, electro-phoretic and immunological techniques (see BOHN, 1976). Enzymatic and hormonal methods are usually specific for proteins of known function or effect, whose nature must, therefore, be defined before the protein can be detected. By contrast, electrophoretic and immunological procedures can be carried out without assumption as to the function of the protein being sought. The broad applicability of electrophoretic and immunological methods is, however, offset by their limited ability to distinguish tissue-specific proteins which exist in low concentration amongst the very wide array of intracellular and extracellular proteins found in virtually all tissues. Thus, immunisation of rabbits with 100-300 mg of protein from extracts of human placenta or human fetal liver yielded precipitating antibodies against the proteins commonly found in adult human serum. After adsorption with solid phase adult human serum protein (AVRAMEAS and TERNYNCK, 1969), only some ten precipitin arcs could be detected by antibody-antigen crossed electrophoresis (AACE). We have used two approaches to reduce the strong antibody response to high concentration serum proteins.

Keywords

Albumin Urea Carbohydrate Electrophoresis MgCl2 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1979

Authors and Affiliations

  • R. G. Sutcliffe
  • B. M. Kukulska
  • L. V. B. Nicholson
  • W. F. Paterson

There are no affiliations available

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