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Drug Product Development for the Back of the Eye pp 47–84Cite as

Fluorophotometry for Pharmacokinetic Assessment

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Part of the book series: AAPS Advances in the Pharmaceutical Sciences Series ((AAPS,volume 2))

Abstract

The corneal epithelium provides a semi-impermeable barrier between the eye and the environment. With continuous intercellular tight junctions, the corneal epithelium exerts a high resistance to passage of ions. Fluorescein staining of the corneal epithelium and stroma has been a subjective measure of the quality of the epithelial barrier. Maurice (1963) was an early pioneer in devising a slit lamp-based instrument to quantify the fluorescein in the cornea of human subjects. Cunha-Vaz (Br J Ophthalmol 59:649-656, 1975) expanded the technology to perform multiple measures of fluorescein concentration as the instrument’s focal plane moved through the eye. Since these early instruments, several commercial fluorophotometers have become available with application to drug delivery of fluorescent tracers.

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Author information

Authors and Affiliations

  1. Emory University School of Medicine, Eye Center, 1365B Clifton Road, Suite B2600, Atlanta, GA, 30322, USA

    Bernard E. McCarey PhD

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  1. Bernard E. McCarey PhD
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Correspondence to Bernard E. McCarey PhD .

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Editors and Affiliations

  1. School of Pharmacy, University of Colorado Denver, East 17th Avenue 12631, Aurora, 80045, Colorado, USA

    Uday B. Kompella

  2. School of Medicine, Emory University, Clifton Road NE., 1365, Atlanta, 30322, Georgia, USA

    Henry F. Edelhauser

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© 2011 American Association of Pharmaceutical Scientists

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McCarey, B.E. (2011). Fluorophotometry for Pharmacokinetic Assessment. In: Kompella, U., Edelhauser, H. (eds) Drug Product Development for the Back of the Eye. AAPS Advances in the Pharmaceutical Sciences Series, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9920-7_3

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