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Hydration Dynamics of Probes and Peptides in Captivity

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Book cover Reviews in Fluorescence 2010

Part of the book series: Reviews in Fluorescence ((RFLU,volume 2010))

Abstract

Water confined on nanometer-length scales is found in many physical and biological environments. Confinement induces special dynamics in liquids, different from that of their bulk counterparts. Reverse micelles, formed by the self-assembly of amphiphilic surfactants in nonpolar solvents, have emerged as an appropriate molecular assembly to monitor the property of water upon confinement due to a number of reasons. A unique advantage of reverse micelles is that molecular dynamics can be monitored with varying states of hydration that is difficult to achieve with assemblies, such as membranes. In this article, we focus on the change in confined hydration dynamics accompanied with increasing hydration, monitored by red edge excitation shift (REES). REES can be effectively used to directly monitor the environment and dynamics around a fluorophore in a molecular assembly utilizing slow solvent relaxation around an excited state fluorophore. It is apparent from the examples discussed that change in solvent relaxation with hydration is complicated and could depend on a number of factors, such as the location of the probe in the reverse micelle and the structure and compactness of the fluorophore involved. We conclude that care should be exercised in interpreting such results.

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Abbreviations

12-AS:

12-(9-Anthroyloxy)stearic acid

2-AS:

2-(9-Anthroyloxy)stearic acid

6-AS:

6-(9-Anthroyloxy)stearic acid

AOT:

Sodium bis(2-ethylhexyl)sulfosuccinate

EGFP:

Enhanced green fluorescent protein

GFP:

Green fluorescent protein

NBD:

7-Nitrobenz-2-oxa-1,3-diazol-4-yl

NBD-cholesterol:

25-[N-[(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-methyl]amino]-27-norcholesterol

NBD-PE:

N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine

REES:

Red edge excitation shift

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Acknowledgments

Work in A.C.’s laboratory was supported by the Council of Scientific and Industrial Research and Department of Science and Technology, Government of India. S.H. thanks the Council of Scientific and Industrial Research for the award of a Senior Research Fellowship. A.C. is an Adjunct Professor at the Special Centre for Molecular Medicine of Jawaharlal Nehru University (New Delhi, India) and Honorary Professor of the Jawaharlal Nehru Centre for Advanced Scientific Research (Bangalore, India). A.C. gratefully acknowledges J.C. Bose Fellowship (Department of Science and Technology, Government of India). Some of the work described in this article was carried out by former members of A.C.’s group whose contributions are gratefully acknowledged. We thank Arunima Chaudhuri for help with Fig. 1 and members of our laboratory for critically reading the manuscript.

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  1. Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad, 500 007, India

    Sourav Haldar & Amitabha Chattopadhyay

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  1. Sourav Haldar
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  2. Amitabha Chattopadhyay
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Correspondence to Amitabha Chattopadhyay .

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  1. Institute of Fluorescence, University of Maryland, E. Pratt St. 701, Baltimore, 21202, Maryland, USA

    Chris D. Geddes

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Haldar, S., Chattopadhyay, A. (2012). Hydration Dynamics of Probes and Peptides in Captivity. In: Geddes, C. (eds) Reviews in Fluorescence 2010. Reviews in Fluorescence, vol 2010. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-9828-6_7

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