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Oncogenomics/Proteomics of Head and Neck Cancers

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Head and Neck Cancer

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Abstract

Head and neck cancer treatment has experienced great advances in surgical techniques, radiation therapy, chemotherapy, and molecular targeted strategies through the years. In addition, there has been explosive growth in our understanding of tumor biology through research focusing on individual genes and their gene products. However, poor overall survival persists despite this progress, in large part because treatment decisions continue to be based on traditional parameters, such as tumor size, tumor site, and presence of regional or distant metastases. Head and neck cancer represents an extremely heterogeneous disease with dysregulation of multiple interrelated cellular pathways, including differentiation, apoptosis, angiogenesis, and metastasis. The complexity of interactions between genes and proteins and the environment and the difficulty of finding the right combinations of targets to study pose fundamental problems with successful identification of therapeutic targets and predictive elements. Oncogenomic and proteomic analyses offer the opportunity to accelerate the pace of discovery for clinically relevant targets. A variety of high-throughput technologies including expression profiling and mass spectrometry technologies are being used to analyze cancer genomes and proteomes with the ultimate goal of identifying new cancer genes and therapeutic targets. Potentially, the identification of disease-associated proteins and protein signatures could be used as tumor markers for early detection, response to therapy, or relapse. A greater understanding of the molecular events underpinning clinical outcomes will provide useful tools in the identification of new targets for future therapy. These advances have already begun to manifest in several key areas of treatment including early detection of cancer, evaluation of surgical margins, determination of necessary extent of surgery, and predictions of outcome and recurrence. In this chapter we review key technological advances leading to these recent changes as well as many of the studies helping to implement these technologies and apply them to patients with head and neck cancer.

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References

  1. Jeon GA, Lee JS, Patel V, et al. Global gene expression profiles of human head and neck squamous carcinoma cell lines. Int J Cancer. 2004;112:249–58.

    Article  PubMed  CAS  Google Scholar 

  2. Ye H, Pungpravat N, Huang BL, et al. Genomic assessments of the frequent loss of heterozygosity region on 8p21.3-p22 in head and neck squamous cell carcinoma. Cancer Genet Cytogenet. 2007;176:100–6.

    Article  PubMed  CAS  Google Scholar 

  3. Jarvinen AK, Autio R, Haapa-Paananen S, et al. Identification of target genes in laryngeal squamous cell carcinoma by high-resolution copy number and gene expression microarray analyses. Oncogene. 2006;25:6997–7008.

    Article  PubMed  Google Scholar 

  4. Jarvinen AK, Autio R, Kilpinen S, et al. High-resolution copy number and gene expression microarray analyses of head and neck squamous cell carcinoma cell lines of tongue and larynx. Genes Chromosom Cancer. 2008;47:500–9.

    Article  PubMed  CAS  Google Scholar 

  5. Mendez E, Cheng C, Farwell DG, et al. Transcriptional expression profiles of oral squamous cell carcinomas. Cancer. 2002;95:1482–94.

    Article  PubMed  CAS  Google Scholar 

  6. Ha PK, Benoit NE, Yochem R, et al. A transcriptional progression model for head and neck cancer. Clin Cancer Res. 2003;9:3058–64.

    PubMed  CAS  Google Scholar 

  7. Chen C, Mendez E, Houck J, et al. Gene expression profiling identifies genes predictive of oral squamous cell carcinoma. Cancer Epidemiol Biomarkers Prev. 2008;17:2152–62.

    Article  PubMed  CAS  Google Scholar 

  8. Ginos MA, Page GP, Michalowicz BS, et al. Identification of a gene expression signature associated with recurrent disease in squamous cell carcinoma of the head and neck. Cancer Res. 2004;64:55–63.

    Article  PubMed  CAS  Google Scholar 

  9. Ziober AF, Patel KR, Alawi F, et al. Identification of a gene signature for rapid screening of oral squamous cell carcinoma. Clin Cancer Res. 2006;12:5960–71.

    Article  PubMed  CAS  Google Scholar 

  10. Kondoh N, Ohkura S, Arai M, et al. Gene expression signatures that can discriminate oral leukoplakia subtypes and squamous cell carcinoma. Oral Oncol. 2007;43:455–62.

    Article  PubMed  CAS  Google Scholar 

  11. Ramaswamy S, Ross KN, Lander ES, Golub TR. A molecular signature of metastasis in primary solid tumors. Nat Genet. 2003;33:49–54.

    Article  PubMed  CAS  Google Scholar 

  12. Schmalbach CE, Chepeha DB, Giordano TJ, et al. Molecular profiling and the identification of genes associated with metastatic oral cavity/pharynx squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 2004;130:295–302.

    Article  PubMed  Google Scholar 

  13. Belbin TJ, Singh B, Smith RV, et al. Molecular profiling of tumor progression in head and neck cancer. Arch Otolaryngol Head Neck Surg. 2005;131:10–8.

    Article  PubMed  Google Scholar 

  14. Roepman P, Wessels LF, Kettelarij N, et al. An expression profile for diagnosis of lymph node metastases from primary head and neck squamous cell carcinomas. Nat Genet. 2005;37:182–6.

    Article  PubMed  CAS  Google Scholar 

  15. Hensen EF, De Herdt MJ, Goeman JJ, et al. Gene-expression of metastasized versus non-metastasized primary head and neck squamous cell carcinomas: a pathway-based analysis. BMC Cancer. 2008;8:168.

    Article  PubMed  Google Scholar 

  16. Rickman DS, Millon R, De Reynies A, et al. Prediction of future metastasis and molecular characterization of head and neck squamous-cell carcinoma based on transcriptome and genome analysis by microarrays. Oncogene. 2008;27:6607–22.

    Article  PubMed  CAS  Google Scholar 

  17. Chung CH, Parker JS, Karaca G, et al. Molecular classification of head and neck squamous cell carcinomas using patterns of gene expression. Cancer Cell. 2004;5:489–500.

    Article  PubMed  CAS  Google Scholar 

  18. Cromer A, Carles A, Millon R, et al. Identification of genes associated with tumorigenesis and metastatic potential of hypopharyngeal cancer by microarray analysis. Oncogene. 2004;23:2484–98.

    Article  PubMed  CAS  Google Scholar 

  19. Weber F, Xu Y, Zhang L, et al. Microenvironmental genomic alterations and clinicopathological behavior in head and neck squamous cell carcinoma. JAMA. 2007;297:187–95.

    Article  PubMed  CAS  Google Scholar 

  20. Ishigami T, Uzawa K, Higo M, et al. Genes and molecular pathways related to radioresistance of oral squamous cell carcinoma cells. Int J Cancer. 2007;120:2262–70.

    Article  PubMed  CAS  Google Scholar 

  21. Torres-Roca JF, Eschrich S, Zhao H, et al. Prediction of radiation sensitivity using a gene expression classifier. Cancer Res. 2005;65:7169–76.

    Article  PubMed  CAS  Google Scholar 

  22. Pramana J, Van den Brekel MW, van Velthuysen ML, et al. Gene expression profiling to predict outcome after chemoradiation in head and neck cancer. Int J Radiat Oncol Biol Phys. 2007;69:1544–52.

    Article  PubMed  CAS  Google Scholar 

  23. Ganly I, Talbot S, Carlson D, et al. Identification of angiogenesis/metastases genes predicting chemoradiotherapy response in patients with laryngopharyngeal carcinoma. J Clin Oncol. 2007;25:1369–76.

    Article  PubMed  CAS  Google Scholar 

  24. Li Y, St John MA, Zhou X, et al. Salivary transcriptome diagnostics for oral cancer detection. Clin Cancer Res. 2004;10:8442–50.

    Article  PubMed  CAS  Google Scholar 

  25. Li Y, Elashoff D, Oh M, et al. Serum circulating human mRNA profiling and its utility for oral cancer detection. J Clin Oncol. 2006;24:1754–60.

    Article  PubMed  CAS  Google Scholar 

  26. Choi P, Chen C. Genetic expression profiles and biologic pathway alterations in head and neck squamous cell carcinoma. Cancer. 2005;104:1113–28.

    Article  PubMed  CAS  Google Scholar 

  27. Yu YH, Kuo HK, Chang KW. The evolving transcriptome of head and neck squamous cell carcinoma: a systematic review. PLoS One. 2008;3:e3215.

    Article  PubMed  Google Scholar 

  28. Soltys SG, Le QT, Shi G, et al. The use of plasma surface-enhanced laser desorption/ionization time-of-flight mass spectrometry proteomic patterns for detection of head and neck squamous cell cancers. Clin Cancer Res. 2004;10:4806–12.

    Article  PubMed  CAS  Google Scholar 

  29. Roesch-Ely M, Nees M, Karsai S, et al. Proteomic analysis reveals successive aberrations in protein expression from healthy mucosa to invasive head and neck cancer. Oncogene. 2007;26:54–64.

    Article  PubMed  CAS  Google Scholar 

  30. Freed GL, Cazares LH, Fichandler CE, et al. Differential capture of serum proteins for expression profiling and biomarker discovery in pre- and posttreatment head and neck cancer samples. Laryngoscope. 2008;118:61–8.

    Article  PubMed  CAS  Google Scholar 

  31. Ralhan R, Desouza LV, Matta A, et al. Discovery and verification of head-and-neck cancer biomarkers by differential protein expression analysis using iTRAQ labeling, multidimensional liquid chromatography, and tandem mass spectrometry. Mol Cell Proteomics. 2008;7:1162–73.

    Article  PubMed  CAS  Google Scholar 

  32. Gourin CG, Zhi W, Adam BL. Proteomic identification of serum biomarkers for head and neck cancer surveillance. Laryngoscope. 2009;119:1291–302.

    Article  PubMed  CAS  Google Scholar 

  33. Choi P, Jordan CD, Mendez E, et al. Examination of oral cancer biomarkers by tissue microarray analysis. Arch Otolaryngol Head Neck Surg. 2008;134:539–46.

    Article  PubMed  Google Scholar 

  34. Sidransky D, Irizarry R, Califano JA, et al. Serum protein MALDI profiling to distinguish upper aerodigestive tract cancer patients from control subjects. J Natl Cancer Inst. 2003;95:1711–7.

    PubMed  CAS  Google Scholar 

  35. Wadsworth JT, Somers KD, Stack Jr BC, et al. Identification of patients with head and neck cancer using serum protein profiles. Arch Otolaryngol Head Neck Surg. 2004;130:98–104.

    Article  PubMed  Google Scholar 

  36. Hu S, Arellano M, Boontheung P, et al. Salivary proteomics for oral cancer biomarker discovery. Clin Cancer Res. 2008;14:6246–52.

    Article  PubMed  CAS  Google Scholar 

  37. Ohshiro K, Rosenthal DI, Koomen JM, et al. Pre-analytic saliva processing affect proteomic results and biomarker screening of head and neck squamous carcinoma. Int J Oncol. 2007;30:743–9.

    PubMed  CAS  Google Scholar 

  38. St John MA, Li Y, Zhou X, et al. Interleukin 6 and interleukin 8 as potential biomarkers for oral cavity and oropharyngeal squamous cell carcinoma. Arch Otolaryngol Head Neck Surg. 2004;130:929–35.

    Article  Google Scholar 

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Authors and Affiliations

  1. Eye & Ear Institute, Suite 500, 200 Lothrop Street, Pittsburgh, PA, 15213, USA

    Jennifer R. Grandis

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  1. Howard S. Moskowitz
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  2. Jennifer R. Grandis
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Correspondence to Jennifer R. Grandis .

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Editors and Affiliations

  1. , Dept of Radio-Oncology, Genolier Swiss Medical Network, 4 Route du Muids, Genolier, CH-1272, Switzerland

    Jacques Bernier

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Moskowitz, H.S., Grandis, J.R. (2011). Oncogenomics/Proteomics of Head and Neck Cancers. In: Bernier, J. (eds) Head and Neck Cancer. Springer, New York, NY. https://doi.org/10.1007/978-1-4419-9464-6_4

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  • DOI: https://doi.org/10.1007/978-1-4419-9464-6_4

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4419-9463-9

  • Online ISBN: 978-1-4419-9464-6

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