Abstract
Carotid body chemotransduction mechanisms involve many neurotransmitters that are synthetized, stored, and released from the chemoreceptor cell. Acetylcholine (ACh) and dopamine (DA) are the main transmitters studied to date. It has been suggested that, at least in cats, ACh is an excitatory whereas DA is an inhibitory transmitter in the carotid body (as review see Eyzaguirre and Zapata, 1884; Fidone et al., 1997; Fitzgerald, 2000). However, their role on chemosensory activity in response to hypoxia is still debated. Recently, in catecholamine-depleted cats where the storage and release of carotid body DA was greatly impeded by the use of ∝-methyl-paratyrosine and reserpine (Bairam and Marchai, in press), DA infiision was showed to inhibit the carotid sinus nerve chemosensory discharge rate (CSND) while ACh reversed the effect under basal condition. In response to hypoxia, DA slowed the initial increase of CSND whereas ACh accelerated it, while neither drug altered the steady-state chemosensory discharge under hypoxic conditions. One interpretation of these results was that DA infusion prevented the expression of ACh excitatory effect during hypoxia as it was infused methyl-paratyrosine maintained throughout ACh administration. Using a similar model of adult cats pre-treated with oc-methyl-paratyrosine and reserpine (Bairam and Marchai, in press), we investigated the effect of 1) ACh infusion on CSND response to different inspiratory oxygen concentrations (Fi02) and 2) DA infusion while ACh is maintained. This protocol of drug administration should reveal the excitatory role of ACh on CSND under basal condition particularly in hypoxia. Indeed, the methyl-paratyrosine with oc-methyl-paratyrosine and reserpine, which inhibits and depletes catecholamine synthesis and storage (Leitner and Roumy, 1986; Fitzgerald et al., 1983), should minimize the effects of endogenous release of DA on CSND.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bairam A., and Marchai F., Carotid sinus nerve chemosensory response to dopamine and acetylcholine in catecholamine depleted cats. Respir. Physiol. Neurobiol. In press.
Docherty, R.J., and McQueen S., 1979, The effects of acetylcholine and dopamine on carotid chemosensory activity in the rabbit. J. Physiol. 288, 411–423.
Eyzaguirre, C, and Zapata,P., 1984, Perspectives in carotid body research. J. Appl. Physiol. 57,931–957.
Fidone, S.J., Gonzalez, C., Almaraz, L., Dinger B., 1997, Cellular mechanisms of peripheral chemoreceptor function. In: The Lung vol. 2, Crystal, R.J., West, J.B., Barnes, P.J., and Weibel, E.R., (eds), Lippincott-Raven, Philadelphia, New York, pp 1725–1746.
Fitzgerald, R.S., 2000, Oxygen and carotid body chemotransduction: the cholinergic hypothesis - a brief history and new evaluation. Respir. Physiol. 120, 89–104.
Fitzgerald, R.S., Garger, P., Hauer, M.C., Raff, H., Fecheter, L., 1983, Effect of hypoxia and hypercapnia on catecholamine content in cat carotid body. J. Appl. Physiol. 54 1408–1413.
Iturriaga, R., Alcayaga, J., Zapata, P., 2000, Lack of correlation between cholinergic-induced changes in chemosensory activity and dopamine release from the cat carotid body in vitro. Brain Res. 868, 380–385.
Leitner, L.M., and Roumy, M., 1986, Chemoreceptor response to hypoxia and hypercapnia in catecholamine depleted rabbit and cat carotid bodies in vitro. Pfluger Arch. 406, 419–423.
Mitchell, J.H., and McCloskey, D.I., 1974, Chemoreceptor responses to sympathetic stimulation and changes in blood pressure. Respir. Physiol. 20, 297–302.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2003 Springer Science+Business Media New York
About this paper
Cite this paper
Bairam, A., Lajeunesse, Y. (2003). Concomitant Effect of Acetylcholine and Dopamine on Carotid Chemosensory Activity in Catecholamine Depleted Cats. In: Pequignot, JM., Gonzalez, C., Nurse, C.A., Prabhakar, N.R., Dalmaz, Y. (eds) Chemoreception. Advances in Experimental Medicine and Biology, vol 536. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-9280-2_44
Download citation
DOI: https://doi.org/10.1007/978-1-4419-9280-2_44
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-4873-3
Online ISBN: 978-1-4419-9280-2
eBook Packages: Springer Book Archive