Chemoreception pp 305-311 | Cite as

Adenosine-Acetylcholine Interactions at the Rat Carotid Body

  • Sílvia V. Conde
  • Emília C. Monteiro
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 536)


Excitatory effects on carotid body (CB) chemotransduction have been described for adenosine and acetylcholine (ACh). The importance of the excitatory effects of exogenous (Monteiro and Ribeiro, 1987) and endogenous adenosine (Monteiro and Ribeiro, 1989) observed in vivo in the rat is supported by the increase in adenosine concentrations during hypoxia (Conde and Monteiro, 2001). In response to hypoxia, the CB also releases ACh (Fitzgerald et al. 1999) that activates nicotinic receptors (Shirahata et ai 1997). ACh and nicotine increase [Ca2+] in type I cells via activation of nicotinic receptors (Dasso et al 1997) and induce the release of several neurotransmitters at the CB, e.g. catecholamines (Obeso et ai 1997). In the present work we tested the hypothesis that ACh can induce the release of adenosine from rat carotid body by a mechanism that involves the activation of nicotinic receptors.


Nicotinic Receptor Carotid Body Nicotinic Agonist Adenosine Concentration Nicotinic Antagonist 
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  1. Alexander, S.P.H., Mathie, A. and Peters, J.A., 2001. TIPS Nomenclature Supplement 20th Edition, 1–12.Google Scholar
  2. Cohen, G., Han, Z.Y., Grailhe, R., Gallego, J., Gaultier, C, Changeux, J.P. and Lagercrantz, H, 2002. p2 nicotinic acetylcholine receptor subunit modulates protective responses to stress: A receptor basis for sleep-disordered breathing after nicotine exposure, PNAS 99:13272–13277.PubMedCrossRefGoogle Scholar
  3. Conde, S.V. and Monteiro, E.C, 2001. Hypoxia and extracellular pathway of adenosine production. Eur. J. Biochem. 268:67.Google Scholar
  4. Conde, S.V. and Monteiro, E.C, 2002. Origin of adenosine released by the rat carotid body. XVth ISAC Symposium. Lyon (France).Google Scholar
  5. Correia-de-S¨¢, P. and Ribeiro, J.A., 1994, Potentiation by tonic A2a-adenosine receptor activation of CGRP-facilitated [3H]- ACh release from rat motor nerve endings, Br.J.Pharmacol., 111: 582–588CrossRefGoogle Scholar
  6. Cunha, R.A., Milusheva, E., Vizi, E.S., Ribeiro, J.A. and Sebastião, A.M., 1994. Excitatory and inhibitory effects of Al and A2 adenosine receptor activation on the electrically evoked [3H] acetylcholine release from different areas of the rat hippocampus. J. Neurochem. 63:207–214.PubMedCrossRefGoogle Scholar
  7. Dasso, L.L.T., Buckler, K.J. and Vaughan-Jones, R.D., 1997. Muscarinic and nicotinic receptors raise intracellular Ca2+ levels in rat carotid body type I cells, J. Physiol. 498:327–338.PubMedGoogle Scholar
  8. Fitzgerald, R.S., Shirahata, M. and Wang, H-Y., 1999. Acetylcholine release from cat carotid bodies, Brain Res., 841:53–61.PubMedCrossRefGoogle Scholar
  9. Ishizawa, Y., Fitzgerald, R.S., Shirahata, M. and Schofïeld, B., 1996. Localization of nicotinic acetylcholine receptors in cat carotid body and petrosal ganglion. Adv. Exp. Med. Biol. 410:253–256.PubMedCrossRefGoogle Scholar
  10. Monteiro, E.C. and Ribeiro, J.A., 1987. Ventilatory effects of adenosine mediated by carotid chemoreceptors in the rat. Naunyn-Schmiedeberg’s Arch. Pharmacol 335:143–148.CrossRefGoogle Scholar
  11. Monteiro, E.C. and Ribeiro, J.A., 1989. Adenosine deaminase and adenosine uptake inhibitors facilitate ventilation in rats. Naunyn-Schmiedeberg’s Arch. Pharmacol 340:230–238.CrossRefGoogle Scholar
  12. Nagayama, T., Matsumoto, T., Kuwakubo, F., Fukushima, Y., Yoshida, M., Suzuki-Kusaba, M., Hisa, H., Kimura, T. and Satoh, S., 1999, role of calcium channels in catecholamine secretion in the rat adrenal gland, J. Physiol 520: 503–512PubMedCrossRefGoogle Scholar
  13. Obeso, A., G¨®mez-Nino, M.A., Almaraz, L., Dinger, B., Fidone, S. and Gonzalez, C, 1997. Evidence for two types of nicotinic receptors in the cat carotid body chemoreceptor cells, Brain Res. 754:298–302.PubMedCrossRefGoogle Scholar
  14. Sabey, K., Paradiso, K., Zhang J. and Henry Steinbach, J., 1999. Ligand binding and activation of rat nicotinic a4p2 receptors stably expressed in HEK293 cells, Mol. Pharmacol 55:58–66.PubMedGoogle Scholar
  15. Sebastião, A.M. and Ribeiro, J.A., 2000, Fine-tuning neuromodulation by adenosine, TIPS 21:341–346PubMedGoogle Scholar
  16. Shirahata, M., Fitzgerald, R.S. and Sham, J.S.K., 1997. Acetylcholine increases intracellular calcium of arterial chemoreceptor cells of adult cats, J. Neurophysiol 78:2388–2395.PubMedGoogle Scholar
  17. Zhang, M., Zhong, H., Vollmer, C. and Nurse, C.A., 2000, Co-release of ATP and ACh mediates hypoxic signaling at rat carotid body chemoreceptors. J. Physiol 525: 143–158PubMedCrossRefGoogle Scholar
  18. Zwart, R., van Kleef, R.G.D.M., Gotti, C, Smulders C.J.G.M. and Vijverberg, H.P.M., 2000. Competitive potentiation of acetylcholine effects on neuronal nicotinic receptors by acethylcholinesterase-inhibiting drugs, J.Neurochem. 75:2492–2500.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Sílvia V. Conde
    • 1
  • Emília C. Monteiro
    • 1
  1. 1.Department of Pharmacology Faculty of Medical SciencesNew University of LisbonLisboaPortugal

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