Role of ROS and NO in Hypoxia-induced Increase in Tyrosine Hydroxylase-messenger RNA in PC12 cells
Paraganglia such as the carotid body and retroperitoneal paraganglia constitute a protective system against the adverse effects of hypoxia by measuring arterial p02 and initiating appropriate reflexes. One of these protective reflexes is release of catecholamines into the blood stream by retroperitoneal paraganglia and the fetal adrenal medulla. Concomitant to this release, catecholamine synthesis is enhanced by hypoxia-induced increase in transcription of the rate-limiting enzyme of catecholamine synthesis, i.e. tyrosine hydroxylase (TH). A tumor cell line derived from a rat adrenal medullary tumor, PC 12 cells, is a suitable model to study this hypoxia-induced increased TH gene transcription (Czyzyk-Krzeska et al. 1994; Kroll and Czyzyk-Krzeska, 1998).
KeywordsPC12 Cell Tyrosine Hydroxylase Carotid Body Slot Blot Catecholamine Synthesis
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