Advertisement

Chemoreception pp 193-199 | Cite as

Role of ROS and NO in Hypoxia-induced Increase in Tyrosine Hydroxylase-messenger RNA in PC12 cells

  • Wolfgang Kummer
  • Brigitte Höhler
  • Alexandra Sell
  • Jörg Hänze
  • Uwe Pfeil
  • Anna Goldenberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 536)

Abstract

Paraganglia such as the carotid body and retroperitoneal paraganglia constitute a protective system against the adverse effects of hypoxia by measuring arterial p02 and initiating appropriate reflexes. One of these protective reflexes is release of catecholamines into the blood stream by retroperitoneal paraganglia and the fetal adrenal medulla. Concomitant to this release, catecholamine synthesis is enhanced by hypoxia-induced increase in transcription of the rate-limiting enzyme of catecholamine synthesis, i.e. tyrosine hydroxylase (TH). A tumor cell line derived from a rat adrenal medullary tumor, PC 12 cells, is a suitable model to study this hypoxia-induced increased TH gene transcription (Czyzyk-Krzeska et al. 1994; Kroll and Czyzyk-Krzeska, 1998).

Keywords

PC12 Cell Tyrosine Hydroxylase Carotid Body Slot Blot Catecholamine Synthesis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Agani, F.H., Puchowicz, M., Chavez, J.C., Pichiule, P., and LaManna, J.C., 2002, Role of nitric oxide in the regulation of HIF-la expression during hypoxia. Am. J. Physiol. Cell Physiol. 283: C178–C186.PubMedGoogle Scholar
  2. Beavis, A.D., and Davatol-Hag, H., 1996, The mitochondrial inner membrane anion channel is inhibited by DIDS. J. Bioenerg. Biomembr. 28: 207–214.PubMedCrossRefGoogle Scholar
  3. Bruick, R.K., and McKnight, S.L., 2001, A conserved family of prolyl-4-hydroxylases that modify HIF. Science 294: 1337–1340.PubMedCrossRefGoogle Scholar
  4. Czyzyk-Krzeska, M.F., Furnari, B.A., Lawson, E.E., and Millhorn, D.E., 1994, Hypoxia increases rate of transcription and stability of tyrosine hydroxylase mRNA in pheochromocytoma (PC 12) cells. J. Biol Chem. 269: 760–764.PubMedGoogle Scholar
  5. Dotsch, J., Hanze, J., Dittrich, K., Demirakca, S., Haberberger, R., and Rascher, W., 1997, Stimulation of neuropeptide Y release in rat pheochromocytoma cells by nitric oxide. Eur. J. Pharmacol. 331: 313–317.PubMedCrossRefGoogle Scholar
  6. Epstein, A.C.R., Gleadle, J.M., McNeill, L.A., Hewitson, K.S., O’Rourke, J., Mole, D.R., Mukherji, M., Metzen, E., Wilson, ML, Dhanda, A., Tian, Y.-M., Masson, N., Hamilton, D.L., Jaakola, P., Barstead, R., Hodgkin, J., Maxwell, P.H., Pugh, C.W., Schofield, C.J., and Ratcliffe, P.J., 2001, C. elegans EGL-9 and mammalian homologs define a family of dioxygenases that regulate HIF by prolyl hydroxylation. Cell 107: 43–54.PubMedCrossRefGoogle Scholar
  7. Haddad, J.J., 2002, Recombinant human interleukin (IL)-lß-mediated regulation of hypoxia-inducible factor-la (HIF-la) stabilization, nuclear translocation, and activation requires an antioxidant/reactive oxygen species (ROS)-sensitive mechanism. Eur. Cytokine Netw. 13: 250–260.PubMedGoogle Scholar
  8. Henrich, M., Hoffmann, K., Konig, P., Gruß, M., Fischbach, T., Goedcke, A., Hempelmann, G., and Kummer, W., 2002, Sensory neurons respond to hypoxia with NO production associated to mitochondria. Mot. Cell. Neurosci. 20: 307–322.CrossRefGoogle Scholar
  9. Hohler, B., Lange, B., Holzapfel, B., Goldenberg, A., Hanze, J., Sell, A., Testan, H., Moller, W., and Kummer, W., 1999, Hypoxic upregulation of tyrosine hydroxylase gene expression is paralleled, but not induced, by increased generation of reactive oxygen species in PC 12 cells. FEBS Lett. 457: 53–56.PubMedCrossRefGoogle Scholar
  10. Ivan, M., Kondo, K., Yang, H., Kim, W., Valiando, J., Ohh, M., Salic, A., Asara, J.M., Lane, W.S., and Kaelin jr., W.G., 2001, HIFa targeted for VHL-mediated destruction by proline hydroxylation: implications for O2 sensing. Science 292: 464–468.PubMedCrossRefGoogle Scholar
  11. Jaakola, P., Mole, D.R., Tian, Y.-M., Wilson, M.I., Gielbert, J., Gaskell, S.J., von Kriegsheim, A., Hebestreit, H.F., Mukherji, M., Schofield, C.J., Maxwell, P.H., Pugh, C.W., and Ratcliffe, P.J., 2001, Targeting of HIF-a to the von-Hippel-Lindau ubiquitylation complex by 02-regulated prolyl hydroxylation. Science 292: 464–468.CrossRefGoogle Scholar
  12. Kroll, S.L., and Czyzyk-Krzeska, M.F., 1998, Role of H2O2 and heme-containing O2 sensors in hypoxic regulation of tyrosine hydroxylase gene transcription. Am. J. Physiol 274: C167- C174.PubMedGoogle Scholar
  13. Kroon, A.M., Vries, H., and Nijhof, W., 1976, Protein synthesis in heart mitochondria: mechanism and metabolic aspects. Acta Cardiol. 31: 1–13.PubMedGoogle Scholar
  14. Lopez-Figueroa, M.O., Caamano, C, Morano, M.I., Ronn, L.C., Akil, H., and Watson, S.J., 2000, Direct evidence of nitric oxide presence within mitochondria. Biochem Biophys. Res. Commun. 272: 129–133.PubMedCrossRefGoogle Scholar
  15. Onoue, S., Endo, K., Yajima, T., and Kashimoto, K., 2002, Pituitary adenylate cyclase activating polypeptide regulates the basal production of nitric oxide in PC12 cells. Life Sci. 71: 205–214.PubMedCrossRefGoogle Scholar
  16. Sandau, KB., Zhou, J., Kietzmann, T., and Briine, B., 2001, Regulation of the hypoxia-inducible factor la by the inflammatory mediators nitric oxide and tumor necrosis factor-a in contrast to desferoxamine and phenylarsine oxide. J. Biol. Chem. 276: 39805–39811.PubMedCrossRefGoogle Scholar
  17. Wang, F., Sekine, H., Kikuchi, Y., Takasaki, C., Miura, C, Heiwa, O., Shuin, T., Fujii-Kurijama, Y., and Sogawa, K., 2002, HIF-la-prolyl-hydroxyiase: molecular target of nitric oxide in the hypoxic signal transduction pathway. Biochem. Biophys. Res. Commun. 295: 657–662.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Wolfgang Kummer
    • 1
  • Brigitte Höhler
    • 1
  • Alexandra Sell
    • 1
  • Jörg Hänze
    • 2
  • Uwe Pfeil
    • 1
  • Anna Goldenberg
    • 1
  1. 1.Institute for Anatomy and Cell BiologyJustus-Liebig-UniversityGiessenGermany
  2. 2.Department of Internal MedicineGiessenJustus-Liebig-UniversityGermany

Personalised recommendations