Chemoreception pp 123-127 | Cite as

The Effect of Methanandamide on Isolated Type I Cells

  • Christopher N. Wyatt
  • Keith J. Buckler
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 536)


Previous work from this laboratory has demonstrated that hypoxia inhibits a TASK-1 like background K+ current in isolated neonatal rat carotid body type I cells. This inhibition leads to membrane depolarisation and voltage gated Ca2+ entry (Buckler, 1997; Buckler et al 2000). Maingret et al (2000) have demonstrated that the endocannabinoid anandamide was a direct and selective blocker of TASK-1. Therefore, in this study we have investigated the actions of anandamide and its analogue methanandamide on isolated type I cells.


Membrane Depolarisation Carotid Body Selective Blocker Isolate Type Arterial Chemoreceptor 
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  1. Buckler KJ, Williams BA, Honore E. 2000, An oxygen-, acid-and anaesthetic-sensitive TASK-like background potassium channel in rat arterial chemoreceptor cells. J. Physiol. ;525 Pt 1:135–42.PubMedCrossRefGoogle Scholar
  2. Buckler KJ, Vaughan-Jones RD. 1998, Effects of mitochondrial uncouplers on intracellular calcium, pH and membrane potential in rat carotid body type I cells. J. Physiol;513 (Pt 3):819–33PubMedCrossRefGoogle Scholar
  3. Maingret F, Patel AJ, Lazdunski M, Honore E. 2001, The endocannabinoid anandamide is a direct and selective blocker of the background K(+) channel TASK-1 EMBO J.;20(l- 2):47–54PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Christopher N. Wyatt
    • 1
  • Keith J. Buckler
    • 1
  1. 1.University Laboratory of PhysiologyOxfordUK

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