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Chemoreception pp 123-127 | Cite as

The Effect of Methanandamide on Isolated Type I Cells

  • Christopher N. Wyatt
  • Keith J. Buckler
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 536)

Abstract

Previous work from this laboratory has demonstrated that hypoxia inhibits a TASK-1 like background K+ current in isolated neonatal rat carotid body type I cells. This inhibition leads to membrane depolarisation and voltage gated Ca2+ entry (Buckler, 1997; Buckler et al 2000). Maingret et al (2000) have demonstrated that the endocannabinoid anandamide was a direct and selective blocker of TASK-1. Therefore, in this study we have investigated the actions of anandamide and its analogue methanandamide on isolated type I cells.

Keywords

Membrane Depolarisation Carotid Body Selective Blocker Isolate Type Arterial Chemoreceptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Buckler KJ, Williams BA, Honore E. 2000, An oxygen-, acid-and anaesthetic-sensitive TASK-like background potassium channel in rat arterial chemoreceptor cells. J. Physiol. ;525 Pt 1:135–42.PubMedCrossRefGoogle Scholar
  2. Buckler KJ, Vaughan-Jones RD. 1998, Effects of mitochondrial uncouplers on intracellular calcium, pH and membrane potential in rat carotid body type I cells. J. Physiol;513 (Pt 3):819–33PubMedCrossRefGoogle Scholar
  3. Maingret F, Patel AJ, Lazdunski M, Honore E. 2001, The endocannabinoid anandamide is a direct and selective blocker of the background K(+) channel TASK-1 EMBO J.;20(l- 2):47–54PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Christopher N. Wyatt
    • 1
  • Keith J. Buckler
    • 1
  1. 1.University Laboratory of PhysiologyOxfordUK

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