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Characterization of a new fatty acid response element that controls the expression of the locust muscle FABP gene

  • Qiwei Wu
  • Weihua Chang
  • Jutta Rickers-Haunerland
  • Tobi Higo
  • Norbert H. Haunerland
Part of the Developments in Molecular and Cellular Biochemistry book series (DMCB, volume 38)

Abstract

In vertebrate and invertebrate muscles, the expression of fatty acid binding proteins (FABP) is induced by long chain fatty acids. To identify the fatty acid response elements that mediate this up-regulation, the gene of the FABP expressed in locust flight muscle was cloned, and its upstream sequences analyzed for potential regulatory elements. Comparison with other muscle FABP promoters revealed the presence of a 19-bp imperfect inverted repeat sequence that contains two hexanucleotide half sites (AGTGGT and ATGGGA), interspersed by 3 nucleotides. The promoter activity was studied with reporter gene constructs in L6 myoblasts, in which H-FABP expression is stimulated by long-chain fatty acids in a similar manner as in adult cardio- myocytes. The 19 bp element, located 180 bp upstream of the transcription start site, was found to be essential for the fatty acid induction of gene expression, and gel shift analysis confirmed that this fatty acid response element is capable of binding nu- clear proteins both from rat myoblasts and locust muscle in the presence of fatty acids. A similar, but reverse sequence that is present upstream of all mammalian H-FABP promoters may modulate the expression of the rat H-FABP gene. (Mol Cell Biochem 239: 173–180,2002)

Key words

fatty acid binding protein fatty acid response element FARE Schistocerca gregaria inverted repeat everted repeat gene regulation gel shift 

Abbreviations

FABP

fatty acid binding protein

L-,A- and H-FABP

liver-,adipocyte and heart-FABP

FARE

fatty acid response element

PPAR

peroxisome proliferator activated receptor

PPRE

peroxisome proliferator response element

DR-1

direct repeat separated by 1 nucleotide

IR-3

inverted repeat separated by 3 nucleotides;

ER-3

everted repeat separated by 3 nucleotides

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Copyright information

© Springer Science+Business Media Dordrecht 2002

Authors and Affiliations

  • Qiwei Wu
    • 1
  • Weihua Chang
    • 1
  • Jutta Rickers-Haunerland
    • 1
  • Tobi Higo
    • 1
  • Norbert H. Haunerland
    • 1
    • 2
  1. 1.Department of Biological SciencesSimon Fraser UniversityBurnabyCanada
  2. 2.Department of Biological SciencesSimon Fraser UniversityBurnabyCanada

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