Fixation of biological samples for SEM, in general, utilizes the same principles and materials as described for preparing samples for TEM in Chapter 1. Osmium tetroxide postfixation can usually be omitted, though samples that have “charging problems” leading to image distortion can frequently benefit from osmication. Subsequent dehydration in an ethanol or acetone series followed by critical point drying, freeze-drying, or drying with one of the chemical techniques described in this section produces a sample suitable for viewing with a conventional high-vacuum SEM. Preparation of samples for ESEM or LV SEM viewing may not require dehydration, drying, and coating protocols used for high-vacuum SEM, while materials destined for microanalytical techniques may necessitate specialized techniques to avoid spurious signal generation.
KeywordsVent Valve Sodium Metaperiodate Aldehyde Fixative Free Aldehyde Group Sight Glass
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